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Crystal structure of Sar1-GDP at 1.7 Å resolution and the role of the NH2 terminus in ER export
It is proposed that the hydrophobic Sar1 NH2-terminal activation/recruitment motif, in conjunction with the α1' helix, mediates the initial steps in COPII coat assembly for export from the ER.
Crystal structure of human factor VIII: implications for the formation of the factor IXa-factor VIIIa complex.
Structural Mechanism of Ring-opening Reaction of Glucose by Human Serum Albumin*
- Yu Wang, Haiyang Yu, Xiaoli Shi, Zhipu Luo, D. Lin, Mingdong Huang
- BiologyJournal of Biological Chemistry
- 16 April 2013
Two protein crystal structures of HSA in complex with either glucose or fructose reveal the presence of linear forms of sugar for both monosaccharides and propose a mechanism for glucose ring opening involving both residues Lys-195 and Lys-199.
Structural Comparison of Allogeneic and Syngeneic T Cell Receptor–Peptide-Major Histocompatibility Complex Complexes
Changes in the crystal structures of the 2C/H-2Kbm3–dEV8 allogeneic complex elucidate structural changes that induce an alloresponse and increase complementarity in the TCR–pMHC interface.
Structure-based Engineering of Species Selectivity in the Interaction between Urokinase and Its Receptor
- Lin Lin, H. Gårdsvoll, Q. Huai, Mingdong Huang, M. Ploug
- Biology, ChemistryThe Journal of Biological Chemistry
- 4 February 2010
The molecular basis underlying this difference is reported by solving the crystal structure for the murine uPA·uPAR complex and it is demonstrated by extensive surface plasmon resonance studies that the kinetic rate constants for this interaction can be swapped completely between these orthologs by exchanging only two residues.
Protein disulfide isomerase capture during thrombus formation in vivo depends on the presence of β3 integrins.
Results indicate that both endothelial and platelet β3 integrins contribute to extracellular PDI binding at the vascular injury site, which is important for platelet thrombus formation and fibrin generation after arteriolar wall injury in live mice.
Parmodulins inhibit thrombus formation without inducing endothelial injury caused by vorapaxar.
It is found that parmodulins target the cytoplasmic face of PAR1 without modifying the ligand-binding site, blocking signaling through Gαq but not Gα13 in vitro and thrombus formation in vivo.
Amine-functionalized lanthanide-doped KGdF4 nanocrystals as potential optical/magnetic multimodal bioprobes.
- Qiang Ju, Datao Tu, Xueyuan Chen
- Biology, ChemistryJournal of the American Chemical Society
- 18 January 2012
Amine-functionalized lanthanide-doped KGdF(4) nanocrystals have been demonstrated to be sensitive time-resolved FRET bioprobes to detect a trace amount of biomolecules such as avidin and to be potential T(1)-MRI contrast agents due to a large longitudinal relaxivity of Gd(3+).
Near-infrared-triggered antibacterial and antifungal photodynamic therapy based on lanthanide-doped upconversion nanoparticles.
An efficient NIR-triggered nano-photosensitizer with promising antifungal and antibacterial efficacy for clinical antimicrobial therapy is revealed.
Crystal Structure of the Michaelis Complex between Tissue-type Plasminogen Activator and Plasminogen Activators Inhibitor-1*
- Lihu Gong, Min Liu, Mingdong Huang
- Biology, MedicineThe Journal of Biological Chemistry
- 31 August 2015
The crystal structure of the tPA·PAI-1 Michaelis complex is determined, which shows significant differences from the structure of its urokinase-type plasminogen activator analogue, the u PA·PAi-1Michaelis complex.