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Cloning and mapping of human PKIB and PKIG, and comparison of tissue expression patterns of three members of the protein kinase inhibitor family, including PKIA.
Two novel members of the human cAMP-dependent protein kinase inhibitor (PKI) gene family, PKIB and PKIG, were cloned. The deduced proteins showed 70% and 90% identity with mouse PKIbeta and PKIgamma…
Cloning and expression analysis of human reticulon 4c cDNA
TheRTN4 gene was mapped to human chromosome 2p14–p13 region by the radiation hybridization (RH) and was named RTN4b, a new gene related to the growth and differentiation of neuroendocrine cell.
Cloning and expressional characterization of a full length cDNA encoding human neuronal protein P17.3
A full-length cDNA with 595bp was isolated from a human fetal brain cDNA library and was found that 32.6% and 15.0% of its amino acids were involved in forming alpha-helix and beta-sheet respecitively.
Cloning, tissue expression pattern, and chromosome localization of human protein kinase Bγ gene
It is a valuable clue for cloning the candidate genes related to prostate cancer; Arrhythmogenic Right Ventricular Dysplasia; Chediak-Higashi, NK cell Deficiency; and Hypoparathyrodism with Short Stature, Mental Retardation and Seizures which have already been mapped in this chromosomal region.
Cloning of a novel human zinc finger protein (ZNF199) cDNA
HTGEKP, which is rather conservative especially in the link region between two zinc finger motifs （TGEKP）
Isolation of 24 novel cDNA fragments from microdissected human chromosome band
Analysis of expression spectrum of cFD14-1 suggested that the cDNA fragments obtained should be used to isolate the genes can not been cloned in 14q24.3 region, and these sequences proved to have some homology with known genes in NCBI database.
Molecular cloning and expression analysis of a novel human cDNA fragment encoding a putative Ser/Thr protein kinase
A novel full-length cDNA encoding a putative serine/threonine kinase has been isolated from a human testis cDNA library and named HUMSTK-1 in view of its degree of homology to members of the Ser/Thr protein kinase family.
Cloning , characterization and mapping of the human ATP 5 E gene , identification of pseudogene ATP 5 EP 1 , and definition of the ATP 5 E motif
A cDNA encoding the ε subunit of human ATP synthase, ATP5E, was isolated from heart, skeletal muscle and spleen cDNA libraries respectively and a new conserved motif composed of 24 residues was defined.
Cloning of human cytokine signal transduction inhibitor genehumSOCS-2
An EST (gb/AA115239) with high identity to the mouse cytokine signal transduction inhibitor genemmSOCS-2 was selected in GenBank EST database by the homologous screening method and it was proved to be novel after NCBl database screening.
Cloning, tissue expression pattern characterization and chromosome localization of human peptide methionine sulfoxide reductase cDNA
It was found that peptide methionine sulfoxide reductase (msrA) can reduce Met(O) to Met and therefore restored the biological function of the oxidized proteins.