Mimi H. Chiang

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PROBLEM Human trophoblast expression of class I human leukocyte antigen (HLA) genes is unique in that there is no classical gene expression, but nonclassical HLA-G is expressed and only by cytotrophoblast cells. This differential expression of classical versus nonclassical class I genes suggests tissue specific regulation. Recently, a negative regulatory(More)
OBJECTIVE To determine by reverse transcription-polymerase chain reaction (PCR) whether human leukocyte antigen (HLA) class I messenger RNA (mRNA) is present in mature human spermatozoa. DESIGN Mature human spermatozoa was isolated from donor semen using a swim-up technique. Total RNA was extracted via guanidinium isothiocyanate-cesium chloride(More)
OBJECTIVE Expression of the histocompatibility antigen HLA-G may be required for appropriate invasion and remodeling of uterine spiral arteries. Inappropriate expression of this antigen may result in failure of invasion, leading to partial placental ischemia and gestational disease. STUDY DESIGN To test the hypothesis that the level of expression of HLA-G(More)
Gene therapy with retroviral mediated gene transfer of the herpes simplex thymidine kinase (HS-tk) gene into a tumor mass confers sensitivity of the tumor cells to ganciclovir (GCV). Tumor-specific immunologic responses may develop following treatment of the primary tumor with retroviral HS-tk and GCV. In the present study we assessed whether GCV treatment(More)
Factors affecting amphotericin A synthesis of Streptomyces nodosus, NDMC-034 were studied. Iron, magnesium and manganese were found to stimulate amphotericin A synthesis at concentrations ranging from 10-100 microM. The optimum inoculum size, and the pH of production medium before sterilization for producing amphotericin A, were found to be near 10% (v/v)(More)
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