Miloŝ Rýc

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The adherence of group B streptococci (GBS) of serotypes Ia, II and III to human vaginal cells was studiedin vitro. The adherence was not dependent on the viability of bacteria; killing of GBS by UV irradiation or glutaraldehyde treatment did not inhibit the adherence. Killing of GBS by heating to 56 °C for 1 h led to a pronounced decrease of adherence,(More)
Adherence of three strains of group A streptococci and their fosfomycin-resistant mutants to HEp-2 tissue culture cells was compared with some cell-surface characteristics,i.e. ultrastructure and hydrophobicity. Among Fosr mutants, both well-adhering and weakly adhering mutants were found. Clonal analysis of the mutants proved their greater stability in the(More)
Binding of fibrinogen to the M protein located on the surface fibrillae of group A streptococci impedes deposition of complement and thus contributes to the virulence of these organisms. We investigated this binding by electron microscopy using postembedding immunogold labeling. Both fibrinogen and its D fragment formed a distinct dense layer in the surface(More)
Analogs (di- and trialanine, tetra- and pentapeptide) to the peptide sequence in Group A streptococcus peptidoglycan were synthetized and were used to inhibit the antipeptide portion of peptidoglycan antibodies. The reactions between these peptidoglycan antibodies and peptidoglycan immunodeterminants on whole cells, isolated cell walls, and peptidoglycans(More)
The number of oxidoreduction sites and the total reducing activityof Proteus vulgaris cells was studied by means of Nitro blue tetrazolium after incubation with two substrates. The second substrate was added after ten minutes’ incubation with the first and the given quantities were determined in both samples. The following systems were used:(More)
The adherence activity oStreptococcus agalactiae (group B) strains is directly dependent on the biological state o. cultures The aim of the present paper was to consider the effect of repeated transfers of cultures alternately on solid and liquid media and the effect of the grow th phase The strains, adhering weakly, strongly and variably to epithelial(More)
The localization of the formic dehydrogenase system was studied by means of nitro-blue tetrazolium, using the ultrathin section technique, in large long bodies ofProteus vulgaris, which form the intermediate stage in regeneration of penicillin-induced spheroplasts. In the electron microscope, empty areas, numbering on an average four per cell section, were(More)