Milan Lstibůrek

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Positive assortative mating (PAM) can enhance the additive genetic variance in a breeding population(BP). This increases the potential for gains in the production population (PP, selected subset of the BP) for recurrent selection programs in forest trees. The assortment of mates can be either: (1) by individual tree rank across the whole BP (PAM), or (2)(More)
Complete pedigree information is a prerequisite for modern breeding and the ranking of parents and offspring for selection and deployment decisions. DNA fingerprinting and pedigree reconstruction can substitute for artificial matings, by allowing parentage delineation of naturally produced offspring. Here, we report on the efficacy of a breeding concept(More)
We developed a deterministic model to optimize DNA fingerprinting effort in the presence of gene flow during the application of Breeding without Breeding. The method considers trait’s heritability, level of gene flow, selection differential, and the proportion of progeny test subjected to fingerprinting (truncation). All the model’s variables individually(More)
The availability and affordability of genetic markers made it possible to estimate quantitative genetic parameters without mating designs' structured pedigree. Here, we compared 4-year height's heritability and individuals' breeding values for a western larch common-garden population of 1,418 offspring representing 15 open-pollinated families from a(More)
Nine microsatellite DNA markers (simple sequence repeats, SSRs) were used to estimate pairwise relationships among 597 Scots pine (Pinus sylvestris) trees as well as to generate a sibship structure for quantitative genetic parameters’ estimation comparison. The studied trees were part of an open-pollinated progeny test of 102 first-generation parents. Three(More)
The minimum-inbreeding (MI) seed orchard layout, formulated originally as a global quadratic assignment problem, was expanded into realistic problem sizes that are often encountered in operational forestry, where two modifications were tested: (1) the merging algorithm of independent MI’s solutions (i.e., smaller blocks) and (2) the extended global(More)
While other investigations have described benefits of positive assortative mating (PAM) for forest tree breeding, the allocation of resources among mates in these studies was either equal or varied, using schemes corresponding only to parental rank (i.e., more resources invested in higher-ranking parents). In this simulation study, family sizes were(More)
Spatial randomization of clones across a seed orchard’s grid is commonly applied to promote cross-fertilization and minimize selfing. The high selection differential attained from advanced-generation breeding programs sets high premier on the genetic gain and diversity delivery from seed orchards, thus clonal allocation is important and even more(More)
An application of “Breeding without Breeding” (BwB) is proposed to uncover or extract genetic information from existing plantations, using pedigree reconstruction and BLUP to predict breeding values and identify genetically superior individuals. The focus is on the use of the methodology at the initiation of an operational breeding program to circumvent the(More)
Original seed orchard algorithm “Optimum Neighborhood Seed Orchard Design” was developed as local heuristics to facilitate the establishment of advanced-generation seed orchards with complex configurations. The scheme leads to uniform spatial distribution among adjacent genetic entries, promoting panmixia. The resulting scheme is thus suitable to(More)
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