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Mechanisms of resistance to five aminoglycoside antibiotics: gentamicin (G), tobramycin (T), netilmicin (N), amikacin (A) and isepamicin (I), were assessed in 16 clinical isolates of Pseudomonas aeruginosa serotype O11, originating from five hospitals in Bratislava. All isolates were in vitro highly resistant to all mentioned aminoglycoside antibiotics (MIC(More)
The pharmacokinetics of gentamicin were studied after intratracheal, inhalation, and iv administration to guinea pigs. Gentamicin injected intratracheally was almost completely systemically available. The comparison of the gentamicin plasma AUCs after inhalation and iv administration showed that an inhalation exposure lasting 10 min resulted in an(More)
Seven amikacin-resistant strains of Enterobacteriaceae isolated in Slovakia and Germany were included in this study. The strains were also resistant in vitro to high levels of gentamicin, tobramycin, netilmicin and isepamicin. Phosphocellulose paper binding assays indicated that resistance to aminoglycosides was due to synthesis of aminoglycoside(More)
Polysaccharide-protein complex (PPC) derived from the cell walls of Candida albicans exerts several pathophysiological effects in laboratory animals similar to the symptoms of shock found at acute disseminated candidiasis. The present study evaluated the presumed fragility of hepatic lysosomes in rats in shock-like state following a single dose of PPC (25(More)
An in vitro evaluation of 32 gentamicin-resistant, Gram-negative bacteria from hygiene-centres in Berne, Switzerland and Bratislava, Czechoslovakia, revealed that 24 strains produced gentamicin-modifying enzymes. Occurrence of acetyltransferases (AAC) was confirmed in 16 strains, adenylyltransferase (AAD) /2''/ was observed in 13 strains while 5 of the(More)
Aminoglycoside antibiotics, such as gentamicin, netilmicin, tobramycin, amikacin, may be inactivated by resistant bacteria producing three types of enzymes: O-nucleotidyltransferases, O-phosphotransferases and N-acetyltransferases. Mechanisms of aminoglycoside resistance were analyzed in clinical isolates of gram-negative bacteria from several regions of(More)
Occurrence and transferability of beta-lactam resistance in 30 multi-resistant Escherichia coli, Klebsiella spp., Enterobacter spp., Pantoea agglomerans, Citrobacter freundii and Serratia marcescens strains isolated from children between 0 and 3 years of age is presented. The strains were resistant to ampicillin (30), cefoxitin (22), cefotaxime (30),(More)
We determined the importance and dissemination of enzymatic mechanisms of aminoglycoside resistance in 239 gentamicin-resistant strains of Gram-negative bacilli isolated in Slovakia during the past decade. Over the past 5 years, the resistance to tobramycin has risen by 1.1% to netilmicin by 27.4%, to amikacin by 78.3%, and resistance to isepamicin was high(More)
Our screening for new antiprotozoal antibiotics as described by Nemecet al.1* has revealed a very high incidence of cultures with antiprotozoal activity in the Fungi imperfecti. Nearly all species of the Fungi imperfecti tested are "predacious fungi" characterized by their ability to trap and kill nematodes and/or protozoa2), Dactylaria lutea Routien,(More)