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The dairy cows at the Estonian Agricultural University appeared to have an extremely low selenium status. The selenium level was 5.6 micrograms/l in whole blood and 3.2 micrograms/l in milk, on average. The blood glutathione peroxidase was consequently extremely low. The effects of organic selenium (selenized yeast) and sodium selenite were compared in a(More)
The growth of common mastitis-causing bacteria in milk was followed by a fluorometric technique based on the release of fluorescent 4-methylumbelliferone (4-MU) from 4-methylumbelliferyl-beta-D-glucuronide by the beta-glucuronidase of bacterial or milk origin. Three of four Escherichia coli strains, all four strains of Streptococcus uberis (4/4) and(More)
Supplementing the feed of selenium-deficient diary cows with selenium (Se)-yeast or selenite at a level of 0.2 p.p.m. induced self-cure of subclinical mastitis; the prevalence of quarters harbouring subclinical mastitis (bacteriological criteria) decreased to about one half during the 8 week supplementation period. Three phenomena became apparent to explain(More)
The potency of selected antibacterials on mastitis-causing Escherichia coli, Streptococcus agalactine and Streptococcus uberis in milk, whey and Iso-sensitest broth (ISB) was compared, based on the suppression of bacterial beta-glucuronidase production. The beta-glucuronidase activity in the samples was analysed by substrate-defined fluorometry where the(More)
Milk from inflamed quarters is high in somatic cells, proteolytic activity and lysosomal enzyme activity. Addition of homologous blood plasma and leukocytes into low cell count milk did not increase the plasmin activity, total proteolytic activity or N-acetyl-beta-D-glucosaminidase (NAGase) as anticipated, even if the reconstituted milk samples were(More)
Beta (beta) and delta (delta)-hemolysin of Staphylococcus aureus strains were cultured in vitro in milk lactoserum (whey) prepared from both healthy and mastitis bovine milk. Production of beta- and delta-hemolysins were detected in 12 out of 50 strains studied. The association between N-acetyl-beta-D-glucosaminidase (NAGase) activity, plasmin activity (PL)(More)
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