Mikio Hayashi

Learn More
To understand the significance of negative charges in the extracellular loops of the inwardly rectifying K(+) (Kir2.1) channel, single-point mutants (D112N, D114N, E125Q, D152E, D152K, D152N, E153D, E153K, and E153Q) and double-point mutants (D112N/D114N and D152N/E153Q) were constructed and transfected into COS-1 and HEK293 cells. All single-point mutants,(More)
Potassium channels regulate excitability, epithelial ion transport, proliferation, and apoptosis. In pancreatic ducts, K(+) channels hyperpolarize the membrane potential and provide the driving force for anion secretion. This review focuses on the molecular candidates of functional K(+) channels in pancreatic duct cells, including KCNN4 (KCa 3.1), KCNMA1(More)
Effects of internal spermine on outward single-channel currents through a strongly inwardly rectifying K(+) channel (Kir2.1) were studied at asymmetrical K(+) concentrations (30 mm external and 150 mm internal K(+)). The current-voltage (I-V) relation for the single channel was almost linear and reversed at -37 ± 3 mV (V(R); n = 19). The channel conductance(More)
Transient receptor potential vanilloid 1 (TRPV1) activation in peripheral sensory nerve is known to be associated with various pain-related diseases, thus TRPV1 has been the focus as a target for drug discovery. In this study, we characterized the pharmacological profiles of(More)
  • 1