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Potato leafroll virus (PLRV) was mechanically transmissible when inocula also contained the umbravirus Pea enation mosaic virus-2 (PEMV-2). In plants infected with PLRV and PEMV-2, PLRV accumulated in clusters of mesophyll cells in both inoculated and systemically infected leaves. No transmissions were obtained by coinoculation with Potato virus Y, Potato(More)
A sequence of 5987 nucleotides is reported for the RNA of potato leafroll luteovirus (PLRV). The sequence contains six large open reading frames, and non-coding regions of 174 nucleotides at the 5' end, 141 nucleotides at the 3' end and 197 nucleotides between two large blocks of coding sequences. The 5' coding region encodes two polypeptides of 28,000(More)
The complete sequence of 9871 nucleotides (nts) of parsnip yellow fleck virus (PYFV; isolate P-121) was determined from cDNA clones and by direct sequencing of viral RNA. The RNA contains a large open reading frame between nts 279 and 9362 which encodes a polyprotein of 3027 amino acids with a calculated M(r) of 336212 (336K). A PYFV polyclonal antiserum(More)
UNLABELLED SUMMARY Taxonomy: PLRV is the type species of the genus Polerovirus, in the family Luteoviridae. Isolates are known from most continents, presumably all spread in potato material derived from the Andean region of South America. Physical properties: PLRV particles are isometric and c. 25 nm in diameter. They contain one major (c. 23 kDa) and one(More)
Northern blot analysis with cDNA probes to RNA-3 (1 kb) of raspberry bushy dwarf virus (RBDV) revealed extensive sequence homology with RBDV RNA-2 (2.2 kb). Nucleotide sequencing showed that RNA-2 contains two large open reading frames (ORFs), of 1074 (5' ORF) and 822 (3' ORF) bases. The 3' ORF is virtually identical in sequence to RNA-3, which encodes the(More)
Potato leafroll luteovirus (PLRV) is transmitted naturally by aphids, but two isolates (15 and V) are known to be only poorly transmissible (PAT); these isolates are also distinct in that their particles lack an epitope present in transmissible (HAT) isolates of PLRV (Tamada et al., Ann. Appl. Biol. 104, 107-116, 1984; Massalski and Harrison, J. Gen. Virol.(More)
Satellite RNA depend for their multiplication on the co-infection of a host cell by a helper virus which can itself multiply independently of the satellite. Four types of satellite RNA have been distinguished on the basis of the size of the RNA and what sort, if any, of protein they encode. One of them, the B-type, comprises relatively large RNA which are(More)