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We have developed a novel technique for combined immunofluorescence/in situ hybridization on fixed budding yeast cells that maintains the three-dimensional structure of the nucleus as monitored by focal sections of cells labeled with fluorescent probes and by staining with a nuclear pore antibody. Within the resolution of these immunodetection techniques,(More)
As the social software market matures, expectations for basic features have converged while vendors innovate with specialized functionality. Buyers need to understand their requirements before looking for solutions. ■ When comparing lists of features, there is a great deal of commonality between the leading vendors of social software in the workplace. ■(More)
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