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Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-ray Photoreduction*
Background: Lytic polysaccharide monooxygenases (LPMOs) exhibit a copper center that binds dioxygen for catalysis. Results: We present LPMO structures from Cu(II) to Cu(I) and analyze the transitionExpand
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Structural and functional studies of the glycoside hydrolase family 3 β-glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii
Cel3A from the thermophilic fungus R. emersonii has proven to be more efficient in the hydrolysis of β-glycosidic linkages than Cel3A from H. jecorina.
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Biochemical Characterization and Crystal Structures of a Fungal Family 3 β-Glucosidase, Cel3A from Hypocrea jecorina*
Background: β-Glucosidases hydrolyze the β-linkage between two adjacent molecules in oligomers of glucose. Results: We report the structure and biochemical characterization of Cel3A from HypocreaExpand
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Evolution and functional characterization of pectate lyase PEL12, a member of a highly expanded Clonostachys rosea polysaccharide lyase 1 family
BackgroundPectin is one of the major and most complex plant cell wall components that needs to be overcome by microorganisms as part of their strategies for plant invasion or nutrition. MicrobialExpand
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Structural studies of a glycoside hydrolase family 3 β-glucosidase from the model fungus Neurospora crassa
The glycoside hydrolase family 3 β-glucosidase Cel3A from Neurospora crassa (NcCel3A) is highly specific for cellobiose. The crystal structure of NcCel3A has been solved to 2.25 Å resolution. TheExpand
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Modified cellobiohydrolase–cellulose interactions following treatment with lytic polysaccharide monooxygenase CelS2 (ScLPMO10C) observed by QCM-D
Abstract The beneficial mechanisms of the lytic polysaccharide monooxygenase CelS2 (ScLPMO10C) from Streptomyces coelicolor on interactions between cellulose and the processive cellulaseExpand
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Production of Ready-To-Use Functionalized Sup35 Nanofibrils Secreted by Komagataella pastoris.
Amyloid nanofibrils are excellent scaffolds for designable materials that can be endowed with biotechnologically relevant functions. However, most of all excellent ideas and concepts that have beenExpand
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Comparative evolutionary histories of fungal proteases reveal gene gains in the mycoparasitic and nematode-parasitic fungus Clonostachys rosea
BackgroundThe ascomycete fungus Clonostachys rosea (order Hypocreales) can control several important plant diseases caused by plant pathogenic fungi and nematodes. Subtilisin-like serine proteasesExpand
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Coupled chemistry kinetics demonstrate the utility of functionalized Sup35 amyloid nanofibrils in biocatalytic cascades
Concerns over the environment are a central driver for designing cell-free enzymatic cascade reactions that synthesize non–petrol-based commodity compounds. An often-suggested strategy that wouldExpand
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Kinetic and molecular dynamics study of inhibition and transglycosylation in Hypocrea jecorina family 3 β-glucosidases
β-Glucosidases enhance enzymatic biomass conversion by relieving cellobiose inhibition of endoglucanases and cellobiohydrolases. However, the susceptibility of these enzymes to inhibition andExpand
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