Mihaela Hillebrand

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The interactions of 3-carboxyphenoxathiin with Bovine Serum Albumin (BSA) and Human Serum Albumin (HSA) have been studied by fluorescence and circular dichroism spectroscopy. The binding of 3-carboxyphenoxathiin quenches the BSA and HSA fluorescence, revealing a 1:1 interaction with a binding constant of about 10(5) M(-1). In addition, according to the(More)
The interaction of kaempferol (kaemp), a natural flavonoid to which antioxidative, anti-inflammatory and cardio-protective biological activities have been attributed, with human serum albumin (HSA), the main in vivo transporter of exogenous substances, was investigated by steady-state, synchronous fluorescence and circular dichroism spectroscopies. The(More)
The factors determining the bioaccumulation of lipophilic compounds in wildlife are often poorly understood, partly because it is difficult to do in vivo experiments with animals such as marine mammals and birds. To evaluate the role of phase I biotransformation in the bioaccumulation process of chlorobornanes (toxaphene), this was studied in in vitro(More)
The behaviour of chlorobiphenyls in marine mammals is best described by a pharmacokinetic model where the blood acts as the central transport compartment between the external environment and a number of peripheral organs, each maintaining a dynamic balance with the concentrations in the blood. Thus, blood samples can be a useful tool in monitoring(More)
The association process of a host-guest system, cyclodextrins (CyD) - 3-carboxycoumarin acid (3CCA) was followed by means of UV-vis, circular dichroism and steady-state fluorescence spectroscopies in buffer solution at pH=1. The experimental data were analyzed in order to get information on the stoichiometry, the equilibrium constants and the geometry of(More)
The interaction between eosin and bovine serum albumin in buffer solution, at pH 7.4 has been studied by means of absorption and emission spectroscopy. Applying the Scatchard model to the absorbance data a non-linear plot was obtained, reflecting a complex process. In the fluorescence spectra, two distinct effects were observed. Upon increasing the(More)
Polychlorinated biphenyl concentrations in brain and liver oystercatchers, Haematopus ostralegis, increased similarly in males and females until sexual maturation. The concentrations were higher in adult males than in subadults, but for females the concentrations were lower in adults than in subadults. Absolute PCB, dieldrin and DDE concentrations were(More)