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Hepatocytes isolated by the collagenase digestive method were transplanted into the spleens of syngeneic rats. Morphology and function of the hepatocytes in the spleen were investigated for 12 to 17 months after transplantation. The transplanted hepatocytes proliferated and reconfigured in the spleen without direct perfusion of portal venous blood and with(More)
Hepatic parenchymal cells which were isolated from rats and transplanted into the spleens of syngeneic rats were evaluated by electron microscopy. Fine structure of the isolated hepatocytes demonstrated that, except for a decrease in glycogen particles and focal proliferation of smooth endoplasmic reticulum, the organelles were morphologically(More)
This study aimed to develop methods for the large-scale preparation of hepatocytes from large animal livers and for the mass cryopreservation of isolated hepatocytes. Isolated hepatocytes were obtained from Beagle dogs weighing around 10 kg by collagenase digestion plus preperfusion with the Ca2+ chelator ethylene glycol bis N,N'-tetraacetic acid. The cell(More)
The liver morphology of fetal hepatic tissue transplanted into an ectopic location was investigated over one year period. Fetal liver fragments prepared from a maternal rat on the 18th or 19th day of pregnancy were injected into the splenic parenchyma of syngeneic rats using a 21 gauge needle. Histologically, the fetal liver did not essentially show any(More)
Intraperitoneal transplantation of hepatocytes encapsulated with calcium alginate gel produced a marked improvement in the survival rate (75%) of rats with D-galactosamine (D-gal)-induced acute hepatic failure (AHF) when compared to the rate of 50% in rats undergoing free hepatocyte transplantation (HCTX). The viability of encapsulated hepatocytes was still(More)
Hepatocellular transplantation into the spleen was investigated as a new attempt in utilizing isolated hepatocytes to compensate for impaired liver function. Present study was undertaken to evaluate morphological and histochemical alterations up to 6 weeks following transplantation in hepatocytes transplanted into the splenic parenchyma. Light microscopic(More)
By utilizing a horseradish peroxidase (HRP) axonal flow technique for retrograde tracing, and electron microscopic techniques, attempts were made in adult Wistar rats to elucidate the central organization of the parasympathetic hepatic innervation. The results were as follows: 1) cells of origin directly innervating the liver were located mainly in the left(More)