Michio Fukuma

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Environmental substances were examined for their effect on interferon induction and delayed type hypersensitivity (DTH) responses in mice. Amaranth, safrole, phenacetin and nicotine suppressed the DTH response, and suppressed the serum interferon titers induced by virus infection. However, they did not affect the interferon titers which were induced by(More)
A high yield of viable single cells was attained from isolated pancreatic islets of adult rat by the sequential treatment with EDTA and Dispase. The percentage of single cells was consistently higher with EDTA-Dispase in comparison with EDTA-trypsin treatment, being 65.8 +/- 7.9% and 36.0 +/- 5.4% respectively, when more than 90% of total islet cells were(More)
Adult human pancreatic tissue was minced and digested with collagenase. Resulting cell clusters, including both exocrine and endocrine components, were suspended in the medium and inoculated into 35 mm Petri dishes. Viable cell clusters became spherical 4 or 5 hr after inoculation and attached to the bottom of the dish within 24 hr. They spread out to form(More)
A series of analogues of N-(cyclohexylcarbonyl)-D-phenylalanine (5) have been synthesized and evaluated for their hypoglycemic activity. Relationships were studied between the activity and the three-dimensional structure of the acyl moiety, which was characterized by high-resolution 1H NMR spectroscopy and MNDO calculations. The role of the carboxyl group(More)
Islet cells of adult rat pancreas, dissociated with EDTA-Dispase, were cultivated in Microtest wells for over 2 months. In our cultures, islet cells were free-floating and cohered with each other to reorganize histotypic aggregates resembling the nondissociated islets. Morphologically, excellent preservation of islet cells in the aggregates was confirmed(More)