Michelle Z. L. Kee

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Recent advances in our understanding of brain function have come from using light to either control or image neuronal activity. Here we describe an approach that combines both techniques: a micromirror array is used to photostimulate populations of presynaptic neurons expressing channelrhodopsin-2, while a red-shifted voltage-sensitive dye allows optical(More)
We have assessed the utility of five new long-wavelength fluorescent voltage-sensitive dyes (VSD) for imaging the activity of populations of neurons in mouse brain slices. Although all the five were capable of detecting activity resulting from activation of the Schaffer collateral-CA1 pyramidal cell synapse, they differed significantly in their properties,(More)
Single cell techniques permit the analysis of cellular properties that are obscured by studying the average behavior of cell populations. One way to determine how gene expression contributes to phenotypic differences among cells is to combine functional analysis with transcriptional profiling of single cells. Here we describe a microfluidic device for(More)
This paper describes a PDMS-based microfluidic device to probe and sort cells based on their response to an external stimulus. The device is capable of interrogating cellular events ranging over broad temporal windows (milliseconds to minutes) at the level of a single cell and then sorting and collecting cells for further analysis, such as mRNA expression(More)
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