Michelle Rowe

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In contrast to molecular chaperones that couple protein folding to ATP hydrolysis, protein disulfide-isomerase (PDI) catalyzes protein folding coupled to formation of disulfide bonds (oxidative folding). However, we do not know how PDI distinguishes folded, partly-folded and unfolded protein substrates. As a model intermediate in an oxidative folding(More)
This paper explores the issues that influence the diffusion of innovation as it relates to the adoption of e-commerce by Small and Medium Enterprises (SMEs). It seeks to identify factors facilitating and inhibiting such adoption across contexts – regional, small city and large city. This analysis is cross cultural so the impact of differing economic and(More)
In eukaryotes, translation termination is performed by eRF1, which recognizes stop codons via its N-terminal domain. Many previous studies based on point mutagenesis, cross-linking experiments or eRF1 chimeras have investigated the mechanism by which the stop signal is decoded by eRF1. Conserved motifs, such as GTS and YxCxxxF, were found to be important(More)
[180--90] unit at the head of the sequence provides the adjustable element to measure T 1 of 1 H attached to 13 C. The inter-sequence relaxation delay was set to 5 s and  values were 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 and 1.0 s to provide intensities for each 1 H resonance correlated to its 13 C in the modified HSQC and example intensity data are shown(More)
Human protein disulphide isomerase (hPDI) is an endoplasmic reticulum (ER) based isomerase and folding chaperone. Molecular detail of ligand recognition and specificity of hPDI are poorly understood despite the importance of the hPDI for folding secreted proteins and its implication in diseases including cancer and lateral sclerosis. We report a detailed(More)
The Fanconi Anemia (FA) DNA repair pathway is essential for the recognition and repair of DNA interstrand crosslinks (ICL). Inefficient repair of these ICL can lead to leukemia and bone marrow failure. A critical step in the pathway is the monoubiquitination of FANCD2 by the RING E3 ligase FANCL. FANCL comprises 3 domains, a RING domain that interacts with(More)
Human PDI b'x (K213-P351) was recombinantly expressed from a pET23 vector with an N-terminal MHHHHHHM tag in E.coli BL21 (DE3) pLysS. Cell were grown using standard minimal media to enable incorporation of single nitrogen or carbon sources for structural biology. These sources (U-13 C-glucose or 15 N ammonium sulphate) can be added in addition to(More)
Prions are propagated in Saccharomyces cerevisiae with remarkable efficiency, yet we know little about the structural basis of sequence variations in the prion protein that support or prohibit propagation of the prion conformation. We show that certain single-amino-acid substitutions in the prion protein Sup35 impact negatively on the maintenance of the(More)