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OBJECTIVE To determine whether the transfer of blastocysts on day 5, developed in sequential culture media, resulted in an increase in implantation rate compared with embryos transferred on day 3. DESIGN Comparative study of embryo culture regimes. SETTING Private practice assisted reproductive technology center. PATIENT(S) Twenty-three patients(More)
OBJECTIVE To determine the relationship between blastocyst score and pregnancy outcome. DESIGN Retrospective review of blastocyst transfer in an IVF clinic. SETTING Private assisted reproductive technology unit. PATIENT(S) 107 patients undergoing blastocyst culture and transfer of two embryos. INTERVENTION(S) Culture of all pronucleate embryos in(More)
Oocyte quality is a key limiting factor in female fertility, yet we have a poor understanding of what constitutes oocyte quality or the mechanisms governing it. The ovarian follicular microenvironment and maternal signals, mediated primarily through granulosa cells (GCs) and cumulus cells (CCs), are responsible for nurturing oocyte growth, development and(More)
OBJECTIVE To determine the levels of metabolites surrounding the human oocyte and embryo in vivo. DESIGN Oviduct and uterine fluids were collected throughout the menstrual cycle. Cumulus cells were collected at oocyte retrieval and their production of metabolites was assessed. Samples were analyzed for pyruvate, lactate, and glucose by microfluorimetry.(More)
Serum anti-Müllerian hormone (AMH) concentration and antral follicle count (AFC) are two increasingly popular static measures used to predict ovarian reserve prior to IVF treatment. While they have been shown to be good predictors of oocyte yield during ovarian stimulation, their status as indicators of oocyte quality and pregnancy rates is currently(More)
The effect of macromolecules on mouse embryo development and viability after culture in sequential media was investigated. It was found that high rates of viable blastocysts could be obtained in the absence of any macromolecule. Blastocyst cell numbers were increased when bovine serum albumin was present in the culture medium, although this benefit was not(More)
The aim of this study was to develop a serum-free culture system that could support high levels of cleavage and blastocyst formation from sheep zygotes developed in vitro. To this end, we investigated the effects on sheep zygote development of amino acids, ammonium, vitamins, and culture of embryos in groups in Synthetic Oviduct Fluid (SOF) medium(More)
The role of amino acids, ethylenediaminetetraacetic acid (EDTA), transferrin, oxygen, glucose, glutamine, taurine and ammonium in CF1 mouse zygote development in culture was examined. Non-essential amino acids and glutamine were shown to alleviate the 2-cell block in culture, and acted in synergy with EDTA to facilitate development to the blastocyst stage.(More)
The presence of ammonium in the culture medium has significant detrimental effects on the regulation of embryo physiology and genetics. Ammonium levels build up linearly over time in the culture medium when media containing amino acids are incubated at 37 degrees C. Ammonium in the culture media significantly reduces blastocyst cell number, decreases inner(More)
OBJECTIVE To identify the cumulus cell gene expression associated with oocyte developmental competence, specifically live birth, after single ET (SET) assisted reproductive technology. DESIGN Retrospective gene expression analysis in human cumulus cells from oocytes that established a pregnancy resulting in live birth versus no pregnancy after SET. (More)