Michelle Lane

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OBJECTIVE To determine whether the transfer of blastocysts on day 5, developed in sequential culture media, resulted in an increase in implantation rate compared with embryos transferred on day 3. DESIGN Comparative study of embryo culture regimes. SETTING Private practice assisted reproductive technology center. PATIENT(S) Twenty-three patients(More)
Oocyte quality is a key limiting factor in female fertility, yet we have a poor understanding of what constitutes oocyte quality or the mechanisms governing it. The ovarian follicular microenvironment and maternal signals, mediated primarily through granulosa cells (GCs) and cumulus cells (CCs), are responsible for nurturing oocyte growth, development and(More)
OBJECTIVE To determine the relationship between blastocyst score and pregnancy outcome. DESIGN Retrospective review of blastocyst transfer in an IVF clinic. SETTING Private assisted reproductive technology unit. PATIENT(S) 107 patients undergoing blastocyst culture and transfer of two embryos. INTERVENTION(S) Culture of all pronucleate embryos in(More)
OBJECTIVE To determine the levels of metabolites surrounding the human oocyte and embryo in vivo. DESIGN Oviduct and uterine fluids were collected throughout the menstrual cycle. Cumulus cells were collected at oocyte retrieval and their production of metabolites was assessed. Samples were analyzed for pyruvate, lactate, and glucose by microfluorimetry.(More)
Serum anti-Müllerian hormone (AMH) concentration and antral follicle count (AFC) are two increasingly popular static measures used to predict ovarian reserve prior to IVF treatment. While they have been shown to be good predictors of oocyte yield during ovarian stimulation, their status as indicators of oocyte quality and pregnancy rates is currently(More)
The aim of this study was to develop a serum-free culture system that could support high levels of cleavage and blastocyst formation from sheep zygotes developed in vitro. To this end, we investigated the effects on sheep zygote development of amino acids, ammonium, vitamins, and culture of embryos in groups in Synthetic Oviduct Fluid (SOF) medium(More)
The amino acid requirements of the preimplantation mouse embryo in culture changes as development proceeds from the zygote to the blastocyst stage. Eagle's non-essential amino acids and glutamine significantly increased cleavage rates during the first four cell cycles, while Eagle's essential amino acids without glutamine did not confer any benefit to(More)
In human in-vitro fertilization (IVF) embryos are routinely transferred to the uterus on day 2 or day 3 of development. Resultant implantation and pregnancy rates are disappointingly low, with only approximately 10% of embryos transferred leading to a live birth. The ability to culture embryos to the blastocyst stage should help to resolve this problem by(More)
OBJECTIVE To vitrify mouse and human blastocysts with use of the cryoloop procedure and to assess subsequent development. DESIGN Controlled study of vitrification of mouse and human blastocysts. SETTING Research department of a private assisted reproductive technology unit. PATIENT(S) Blastocysts that were not suitable to be frozen were donated from(More)
Obesity is highly prevalent, and its incidence is increasing. The previous study showing a major effect of paternal obesity on metabolic health of offspring is confounded by comorbidity with diabetes. Therefore, we investigated the effect of diet-induced paternal obesity, in the absence of diabetes, on the metabolic health of two resultant generations and(More)