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Neisseria gonorrhoeae is difficult to cultivate in liquid medium. Currently there are no liquid media, defined or undefined, that reliably permit growth of this bacterium from low inocula. Standard clinical laboratory broths may allow multiplication of some strains of gonococci from large inocula, but such media incorporate infusates, extracts or digests(More)
BACKGROUND Vaginal lactobacilli protect the female genital tract by producing lactic acid, bacteriocins, hydrogen peroxide or a local immune response. In bacterial vaginosis, normal lactobacilli are replaced by an anaerobic flora and this may increase susceptibility to Neisseria gonorrhoeae, a facultative anaerobe. Bacterial interference between vaginal(More)
We determined the rates of survival of six clinical and two control strains of Neisseria gonorrhoeae in six commercial transport systems, using NCCLS standard M40-A methodology. Differences in strain recovery and system performance were marked. A strain less robust than the recommended ATCC 43069 would provide a more exacting standard.
The survival in transport media of 30 strains of gonococci was determined. These strains comprised five distinguishable strains of each of six auxotypes. Survival of different auxotypes varied significantly, but this variability was reduced by charcoal. Delayed swab processing could yield isolates unrepresentative of those causing infection in a sampled(More)
Hepatocyte transplantation is being used in patients with liver-based metabolic disorders and acute liver failure. Hepatocytes are isolated from unused donor liver tissue under GMP conditions. Cells must be free of microbiological contamination to be safe for human use. The experience of microbiological screening during 72 hepatocyte isolation procedures at(More)
Neisseria gonorrhoeae will grow in an anaerobic atmosphere if provided with nitrite as a terminal electron acceptor, and it is increasingly apparent that this important pathogen may grow anaerobically in vivo. By modifying a previously described chemically-defined medium we have produced a liquid medium capable of supporting growth of N. gonorrhoeae under(More)
We report the first detection of a planetary-mass companion to a star using the High Resolution Spectrograph (HRS) of the Hobby-Eberly Telescope (HET). The HET-HRS now gives routine radial velocity precision of 2-3 m s −1 for high SNR observations of quiescent stars. The planetary-mass companion to the metal-rich K0V star HD 37605 has an orbital period of(More)
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