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The recent revelation that the human genome contains only ~30,000 genes underscores the importance of gene regulation in generating organismal diversity. Cis-regulatory DNAs, or enhancers, are short stretches of DNA-300 bp to 1,000 bp in length-that control gene expression. This DNA accounts for a substantial fraction of metazoan genomes, but is largely(More)
Metazoan genomes contain vast tracts of cis-regulatory DNA that have been identified typically through tedious functional assays. As a result, it has not been possible to uncover a cis-regulatory code that links primary DNA sequences to gene expression patterns. In an initial effort to determine whether coordinately regulated genes share a common "grammar,"(More)
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to(More)
The maternal Dorsal regulatory gradient initiates the differentiation of several tissues in the early Drosophila embryo. Whole-genome microarray assays identified as many as 40 new Dorsal target genes, which encode a broad spectrum of cell signaling proteins and transcription factors. Evidence is presented that a tissue-specific form of the NF-Y(More)
Bioinformatics methods have identified enhancers that mediate restricted expression in the Drosophila embryo. However, only a small fraction of the predicted enhancers actually work when tested in vivo. In the present study, co-regulated neurogenic enhancers that are activated by intermediate levels of the Dorsal regulatory gradient are shown to contain(More)
  • Bryce Daines, Hui Wang, Yumei Li, Yi Han, Richard Gibbs, Rui Chen +78 others
  • 2009
Using RNA interference to identify specific modifiers of a temperature-sensitive, embryonic-lethal mutation in the Caenorhabditis elegans ubiquitin-like Nedd8 protein modification pathway E1-activating gene rfl-A screen for modifiers of notch signaling uncovers Amun, a protein with a critical role in sensory organ development .. Analysis of Pax6 contiguous(More)
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