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The existence of GABAergic neurons in the rat suprachiasmatic nucleus (SCN) was demonstrated by three specific markers; mRNA coding for glutamic acid decarboxylase (GAD) and visualized by in situ hybridization using a 35S-labelled cDNA probe, and GAD protein and GABA were identified by immunocytochemistry using specific antisera. In situ hybridization(More)
Dopamine (DA)-containing projections to the cerebral cortex are considered to play an important role in cognitive processes. Using a recently developed monoclonal antiserum directed against DA and an antibody directed against tyrosine hydroxylase in combination with Golgi impregnation and electron microscopy, we have observed that DA and tyrosine(More)
Peroxidase-antiperoxidase electron microscope immunocytochemistry with an antiserum against noradrenaline-glutaraldehyde-protein conjugate was used to identify cortical noradrenaline terminals (axonal varicosities) from the upper layers of the frontal, parietal and occipital cortex in adult rat. A large number of immunostained varicosities were examined in(More)
The aim of the present study is to examine whether aromatic L-amino acid decarboxylase (AADC) catalyzes the conversion of exogenous L-3,4-dihydroxyphenylalanine (L-DOPA) to dopamine in serotonin neurons of the rat dorsal raphe nucleus. First, in order to confirm the localization of AADC in central serotonin neurons, we used an immunoperoxidase method for(More)
An antiserum that has been raised against glutaraldehyde-conjugated dopamine was used to demonstrate specifically dopamine in the rat hypothalamus. This dopamine antiserum permitted an optimal fixation with glutaraldehyde and therefore enabled the simultaneous light and electron microscopic immunocytochemical localization of dopamine. It was demonstrated(More)
We have quantified the density of serotonin axonal varicosities, their synaptic incidence and their distribution among potential targets in the pars reticulata and pars compacta of the rat substantia nigra. Serotonin axonal varicosities, counted at the light microscopic level following in vitro [3H]serotonin uptake and autoradiography, amounted to 9 x(More)
The aim of the present study is to examine whether serotonergic terminals of the rat brain are induced to contain dopamine by L-DOPA administration. In rats that received intraperitoneally L-DOPA plus a peripheral decarboxylase inhibitor, we showed by use of a double-labeling immunofluorescence method that dopamine was localized in serotonergic fibers of(More)
Dopamine was coupled to bovine serum albumin (BSA) with glutaraldehyde, precautions were taken in order to preserve the catechol ring. After injection of this immunogen into rabbits, anti-dopamine antibodies were obtained and tested using radioimmunochemical binding studies and adsorption to catecholamine covered sepharose beads. A good correlation was(More)
gamma-Aminobutyric acid (GABA)-containing elements have been studied by light and electron microscopy in the rat spinal cord, using immunocytochemistry with anti-GABA antibodies. Light microscopy showed immunoreactive somata localized principally in laminae I-III, and occasionally in the deeper laminae of the dorsal horn and in the ventral horn. Small(More)
Converging data suggest that nitric oxide (NO) production by cytokine-induced immune cells in demyelinating lesions is involved in multiple sclerosis (MS). High levels of NO may complex to suitable amino acids, causing an immune response against the formed neo-epitopes. By testing MS sera with chemically defined nitroso-amino acids conjugated to carrier(More)