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MCP/CD46 is a widely distributed C3b/C4b binding regulatory glycoprotein of the complement system that has been identified on all human peripheral blood cells except erythrocytes. In this paper, we describe the identification of bovine CD46 on all blood cells, including erythrocytes, with the newly prepared monoclonal antibody IVA-520. This antibody(More)
Recent studies brought the evidences that some cell surface molecules associated with immune system (cluster of differentiation (CD) molecules) may be involved in the fertilization process. The experimental observations regarding the function of CD9, CD49f/CD29, CD46 and CD11b/CD18 have led to the construction of general hypothesis of fertilization(More)
Membrane cofactor protein (CD46) is complement regulatory protein with probable function in the reproduction process. Expression of CD46 on human, mice, rat and guinea pig spermatozoa is restricted to the inner acrosomal membrane. In spite of the presence of anti-sperm antibodies and other potential complement activating agents in follicular fluid, CD46 is(More)
Sperm-egg interaction and fusion represent a key moment of fertilization. In mammals, it is not possible without the interaction of the tetraspanin superfamily proteins including CD81. A detailed immunohistochemical localization of CD81 was monitored in bovine oocytes during different maturation stages, as well as during early embryogenesis. In addition,(More)
The cattle V antigen from the FV blood group system was characterized. Hemolytic as well as immunochemical analyses with monoclonal antibody (MAb) IVA-41 found that V antigen of bovine red blood cells is a membrane-bound, papain- and pronase-sensitive, trypsin- and chymotrypsin-resistant N-glycosylated sialoglyco-protein with molecular weight of 64, 56, and(More)
The objective of this research was to study the expression of cell membrane molecules CD9 and CD41/61 of transgenic rabbit with integrated human factor VIII (rhFVIII) gene construct. The expressions of these molecules have been monitored during two lactations of transgenic rabbits and simultaneously compared with the expression of the same molecules of(More)
Monoclonal antibody IVA-285 (IgG1 isotype) recognizing antigenic determinant on bovine and ovine immunoglobulin light chain was produced and characterized. Western blot analysis of bovine immunoglobulin G (IgG) and immunoglobulin M (IgM) purified from bovine blood serum as well as whole immunoglobulin fractions of bovine and ovine serum with IVA-285 showed(More)
Artificial insemination with frozen-thawed spermatozoa is commonly used in cattle breeding. A simple and fast procedure is needed for routine evaluation of the acrosomal status of frozen-thawed bovine sperm. Therefore, the purpose of this study was to test two staining procedures used to determine the viability and integrity of acrosome of frozen-thawed(More)
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