Michal Simon

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The mouse monoclonal antibody HNK-1 and the human monoclonal IgM antibody present in patients with polyneuropathy both recognize carbohydrate epitope(s) on human myelin-associated glycoprotein and P0. In the present study, the oligosaccharide structures that bear the antibody epitope(s) were investigated. The extracellular derivative of myelin-associated(More)
MCP/CD46 is a widely distributed C3b/C4b binding regulatory glycoprotein of the complement system that has been identified on all human peripheral blood cells except erythrocytes. In this paper, we describe the identification of bovine CD46 on all blood cells, including erythrocytes, with the newly prepared monoclonal antibody IVA-520. This antibody(More)
Monoclonal antibody IVA-285 (IgG1 isotype) recognizing antigenic determinant on bovine and ovine immunoglobulin light chain was produced and characterized. Western blot analysis of bovine immunoglobulin G (IgG) and immunoglobulin M (IgM) purified from bovine blood serum as well as whole immunoglobulin fractions of bovine and ovine serum with IVA-285 showed(More)
The prevalence and risk factors associated with leptospirosis were studied in veterinary students in Zaragoza. Sera were collected at the beginning and end of the academic year 1994 to 1995 and were tested by ELISA against a pool of Leptospira interrogans serovars bratislava, canicola, grippotyphosa, hardjo, icterohaemorrhagiae and pomona antigens. At the(More)
The epitope for HNK-1 and patient's monoclonal autoantibodies in demyelinating polyneuropathy associated with immunoglobulin M gammopathy is borne by different types of N-linked oligosaccharide structures in human P0 and myelin-associated glycoprotein (MAG). Fourteen glycopeptide fractions bearing different oligosaccharide structures were obtained from(More)
The N-linked oligosaccharide structures of human myelin-associated glycoprotein (MAG) and P0 have been characterized by serial lectin affinity chromatography (SLAC) of 14C-glycopeptides. 14C-Glycopeptides were prepared from purified MAG derivative and P0 by extensive proteolytic digestion and N-14C-acetylation. Assuming that all the 14C-glycopeptides were(More)
Linkage of the main histocompatibility complex (MHC) and the J blood locus was demonstrated in pgis by means of segregation data in families of double back-cross matings. A recombination frequency of 9.82% was estimated. No evidence of close association with the blood group systems A, B, D, E, F, G, H, I, K, L, M, N and O was found.
Germinal vesicle breakdown (GVBD) of bovine oocytes was completely blocked by cycloheximide added to culture medium at concentrations of 1-20 micrograms/ml. Nevertheless, under such conditions a certain degree of chromatin condensation inside the germinal vesicle was observed. The inhibitory effect was not influenced by the presence or absence of cumulus(More)
Recent studies brought the evidences that some cell surface molecules associated with immune system (cluster of differentiation (CD) molecules) may be involved in the fertilization process. The experimental observations regarding the function of CD9, CD49f/CD29, CD46 and CD11b/CD18 have led to the construction of general hypothesis of fertilization(More)
We report on an experimental study of adsorption isotherm of nitrogen onto porous silicon with noninterconnected pores open at one or at both ends in order to check for the first time the old (1938) but always current idea based on Cohan's description which suggests that the adsorption of gas should occur reversibly in the first case and irreversibly in the(More)