Michael Wiedmann

3Patrick Bulau
3Katrin Bomans
3Katharina Diepold
3Michael Mølhøj
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The degradation of proteins by asparagine deamidation and aspartate isomerization is one of several chemical degradation pathways for recombinant antibodies. In this study, we have identified two solvent accessible degradation sites (light chain aspartate-56 and heavy chain aspartate-99/101) in the complementary-determining regions of a recombinant IgG1(More)
  • Markus Haberger, Katrin Bomans, Katharina Diepold, Michaela Hook, Jana Gassner, Tilman Schlothauer +12 others
  • 2014
Modifications like asparagine deamidation, aspartate isomerization, methionine oxidation, and lysine glycation are typical degradations for recombinant antibodies. For the identification and functional evaluation of antibody critical quality attributes (CQAs) derived from chemical modifications in the complementary-determining regions (CDRs) and the(More)
Robust knowledge consists of both conceptual and procedural knowledge. In order to address both types of knowledge, offering students opportunities to explore target concepts in an exploratory learning environment (ELE) is insufficient. Instead, we need to combine exploratory learning environments, to support students acquisition of conceptual knowledge,(More)
This paper describes the development and evaluation of an affect-aware intelligent support component that is part of a learning environment known as iTalk2Learn. The intelligent support component is able to tailor feedback according to a student's affective state, which is deduced both from speech and interaction. The affect prediction is used to determine(More)
  • Katrin Bomans, Antje Lang, Veronika Roedl, Lisa Adolf, Kyrillos Kyriosoglou, Katharina Diepold +9 others
  • 2013
Biotherapeutics are often produced in non-human host cells like Escherichia coli, yeast, and various mammalian cell lines. A major focus of any therapeutic protein purification process is to reduce host cell proteins to an acceptable low level. In this study, various E. coli host cell proteins were identified at different purifications steps by HPLC(More)
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