Michael Wettern

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Genes for glycolytic and Calvin-cycle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of higher eukaryotes derive from ancient gene duplications which occurred in eubacterial genomes; both were transferred to the nucleus during the course of endosymbiosis. We have cloned cDNAs encoding chloroplast and cytosolic GAPDH from the early-branching photosynthetic(More)
Ubiquitin, a highly conserved 76-amino-acid protein, is involved in the response of many types of eukaryotic cells to stress but little is known about its role in lower plants. In the present study we have investigated the distribution of ubiquitin in the unicellular alga Chlamydomonas reinhardii as well as the effect of heat and light stress on its(More)
A detailed characterization of Chlamydomonas reinhardii cDNAs encoding ubiquitin 52-amino-acid fusion proteins is presented in this study. While two cDNAs (designated UBI1 and UBI3) encode the complete ubiquitin extension protein, the third one (UBI2) lacks a minor part of the 5' region as well as a poly(A) tail. Differences between UBI1 and UBI3 are(More)
Isolated thylakoid membranes of Chlamydomonas reinhardi Y-1 with the 32-kDa polypeptide either radioactively labelled or unlabelled were incubated in vitro under various conditions in order to gain information about the degradation of the 32-kDa polypeptide. The degradation was higher at pH 6 compared with pH 7 and pH 8 and exhibited a temperature maximum(More)
Using a new method for the isolation of released mother cell walls of Chlorella fusca, the biosorption of cadmium, copper and lead by purified cell wall isolates and whole cell suspensions was comparatively characterized. In all cases whole cells accumulated more metal ions than isolated cell walls. Both the Langmuir and Freundlich isotherm models were(More)
Cells of Chlamydomonas reinhardtii were synchronized by a light/dark illumination cycle of 14:10 h. All cells divided within the first 2 h of the dark period, the synchronization index was calculated as 0.916. RNA was isolated every 2 h and hybridized to 32P-labeled probes encoding (i) ubiquitin from Chlamydomonas reinhardtii (UBM) and (ii) two different(More)
Applying different stresses (heat shock, photoinhibition, and chilling) to the unicellular green alga Chlamydomonas reinhardtii led to a characteristic transcription pattern of mRNAs encoding ubiquitin extension proteins [0.7-kb transcript(s)] as well as polyubiquitin (2.3-kb transcript). Heat shock resulted in an increase in the amount of polyubiquitin(More)
Hochschulen sind wie Einrichtungen der Privatwirtschaft zu datenschutzkonformem Handeln verpflichtet — die rechtlichen Voraussetzungen dazu sind vorhanden. An ausgewählten Beispielen wird der nicht unbeträchtliche Nachholbedarf beschreiben, den Hochschulen aufzuarbeiten haben, um den Datenschutz als Querschnittsaufgabe in allen Bereichen zu nutzen und ihn(More)