Michael V. Boland

Neil R Miller6
Harry A Quigley6
Prem S Subramanian4
Pradeep Y Ramulu3
6Neil R Miller
6Harry A Quigley
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The new field of location proteomics seeks to provide a comprehensive, objective characterization of the subcellular locations of all proteins expressed in a given cell type. Previous work has demonstrated that automated classifiers can recognize the patterns of all major subcellular organelles and structures in fluorescence microscope images with high(More)
Determination of the functions of all expressed proteins represents one of the major upcoming challenges in computational molecular biology. Since subcellular location plays a crucial role in protein function, the availability of systems that can predict location from sequence or high-throughput systems that determine location experimentally will be(More)
— We describe a method of classifying cellular protein localization patterns based on their appearance in fluorescence light microscope images. Images depicting cellular protein localization were obtained using immunoflu-orescence microscopy. After collection, the images were processed and subject to feature extraction. Zernike moments were calculated for(More)
Purpose. To compare optic disc topography performed by confocal laser ophthalmoscopy in eyes with nonarteritic anterior ischemic optic neuropathy (NAION), arteritic anterior ischemic optic neuropathy (AAION), and open-angle glaucoma (OAG), adjusting for the amount of retinal ganglion cell (RGC) loss, as measured by nerve fiber layer (NFL) thickness and(More)
BACKGROUND The definitive diagnosis of glaucoma is currently based on congruent damage to both optic nerve structure and function. Given widespread quantitative assessment of both structure (imaging) and function (automated perimetry) in glaucoma, it should be possible to combine these quantitative data to diagnose disease. We have therefore defined and(More)
BACKGROUND The concept of risk thresholds has been studied in medical decision making for over 30 years. During that time, physicians have been shown to be poor at estimating the probabilities required to use this method. To better assess physician risk thresholds and to more closely model medical decision making, we set out to design and test a method that(More)
BACKGROUND Understanding how patients move through outpatient clinics is important for optimizing clinic processes. This study compares the costs, benefits, and challenges of two clinically important methods for measuring patient flow: (1) a commercial system using infrared (IR) technology that passively tracks patient movements and (2) a custom-built, low(More)
PURPOSE To assess the relationship between the pupillary light reflex (PLR) and visual field (VF) mean deviation (MD) and retinal nerve fiber layer (RNFL) thickness. METHODS A total of 148 patients with glaucoma (mean age 67 ± 11, 49% female) and 71 controls (mean age 60 ± 10, 69% female) were included in this study. Using a pupillometer, we recorded and(More)
PURPOSE To develop and validate an associative model using pupillography that best discriminates those with and without glaucoma. DESIGN A prospective case-control study. METHODS We enrolled 148 patients with glaucoma (mean age 67 ± 11) and 71 controls (mean age 60 ± 10) in a clinical setting. This prototype pupillometer is designed to record and(More)
PURPOSE To determine risk factors for glaucoma in a population-based study in the United States. METHODS Participants age 40 and older from the National Health and Nutrition Examination Survey underwent questionnaires, physical examination, laboratory tests, and vision tests including fundus imaging. Glaucoma was determined based on expert grading of(More)