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MOTIVATION Assessment of protein subcellular location is crucial to proteomics efforts since localization information provides a context for a protein's sequence, structure, and function. The work described below is the first to address the subcellular localization of proteins in a quantitative, comprehensive manner. RESULTS Images for ten different(More)
Decades of epidemiologic research into open-angle glaucoma have elucidated several risk factors related in some way to the disease. As more and more risk factors are identified, however, assessing their individual and collective contributions in a particular patient is becoming increasingly complicated. To help organize our knowledge of risk in glaucoma we(More)
OBJECTIVE To assess the current state of electronic health record (EHR) use by ophthalmologists, including adoption rate and user satisfaction. DESIGN Population-based, cross-sectional study. PARTICIPANTS A total of 592 members of the American Academy of Ophthalmology (AAO) participated. METHODS A total of 3796 AAO members were randomly selected on(More)
BACKGROUND Glaucoma is an acquired degeneration of the optic nerve and a leading cause of blindness worldwide. Medical and surgical treatments that decrease intraocular pressure may prevent visual impairment and blindness. PURPOSE To compare the effectiveness of medical, laser, and surgical treatments in adults with open-angle glaucoma with regard to(More)
PURPOSE To determine risk factors for glaucoma in a population-based study in the United States. METHODS Participants age 40 and older from the National Health and Nutrition Examination Survey underwent questionnaires, physical examination, laboratory tests, and vision tests including fundus imaging. Glaucoma was determined based on expert grading of(More)
PURPOSE To measure choroidal thickness and to determine parameters associated with it. DESIGN Cross-sectional study. PARTICIPANTS Seventy-four glaucoma patients and glaucoma suspects. METHODS Spectral domain optical coherence tomography (SD-OCT) scans were obtained to estimate average choroidal thickness in a group of glaucoma suspects and glaucoma(More)
Determination of the functions of all expressed proteins represents one of the major upcoming challenges in computational molecular biology. Since subcellular location plays a crucial role in protein function, the availability of systems that can predict location from sequence or high-throughput systems that determine location experimentally will be(More)
The new field of location proteomics seeks to provide a comprehensive, objective characterization of the subcellular locations of all proteins expressed in a given cell type. Previous work has demonstrated that automated classifiers can recognize the patterns of all major subcellular organelles and structures in fluorescence microscope images with high(More)
— We describe a method of classifying cellular protein localization patterns based on their appearance in fluorescence light microscope images. Images depicting cellular protein localization were obtained using immunoflu-orescence microscopy. After collection, the images were processed and subject to feature extraction. Zernike moments were calculated for(More)
OBJECTIVE To determine the extent of difference between better-eye visual field (VF) mean deviation (MD) and integrated VF (IVF) MD among Salisbury Eye Evaluation (SEE) subjects and a larger group of glaucoma clinic subjects and to assess how those measures relate to objective and subjective measures of ability/performance in SEE subjects. DESIGN(More)