Michael-Shawn Fletcher

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The influence of Na+, Ca2+, La3+, and Fe3+ on the adhesion of Pseudomonas fluorescens H2 and H2S was investigated with interference reflection microscopy (IRM). IRM is a light microscopy technique which allows (i) visualization of the adhesive sites of living bacteria as they attach to a glass cover slip surface and (ii) evaluation of the bacterium-glass(More)
 Typhoid fever remains an important health threat in many parts of the world, with an estimated 16 million cases and 600 000 deaths occurring each year. The emergence of Salmonella typhi strains multiply resistant to antibiotics has complicated the treatment of this disease. Field experience of 8 years shows that a vaccine composed of purified Vi capsular(More)
The attachment of bacteria to solid surfaces is influenced by substratum chemistry, but to determine the mechanistic basis of this relationship, homogeneous, well-defined substrata are required. Self-assembled monolayers (SAMs) were constructed from alkanethiols to produce a range of substrata with different exposed functional groups, i.e., methyl and(More)
The prevailing view in the palaeoclimate literature of the last 20 years is that the Southern Westerly Winds (SWW) were intensified over southern Australia and Tasmania during the warmer-than-present early Holocene (11e8 ka). At similar latitudes on the opposite side of the southern mid-latitudes, palaeoclimate studies have suggested a poleward shift of the(More)
Advances in the development and application of investigative tools, such as scanning confocal laser microscopy and genetic techniques, are leading to new insights concerning the composition, structure, and function of bacterial biofilms. Molecular biology has also demonstrated that bacteria are able to 'sense' surface environments, altering their pattern of(More)
Sites of close contact between gliding Cytophaga sp. strain U67 cells and glass were examined by interference reflection microscopy. Site patterns changed during translocation and moved relative to the substratum, in contrast to previous interference reflection microscopy observations of fibroblast and amoeboid motility. Sinistral rotation around the long(More)