Michael Schilling

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A novel electrospray interface is presented which induces an electric field by dielectric polarization through a non-conductive barrier. Therefore, a square-wave high-voltage signal is applied. This technique allows mass spectrometric measurements in the positive as well as in the negative mass spectrometry mode without changing the polarity of the(More)
Immobilized metal affinity chromatography (IMAC) based on Fe (3+) or Ga (3+) chelation is the most widely employed technique for the enrichment of phosphopeptides from biological samples prior to mass spectrometric analysis. An IMAC resin geared mainly toward phosphoprotein enrichment, Pro-Q Diamond, has been assessed for its utility in phosphopeptide(More)
In this work, the combined use of desorption by a continuous wave near-infrared diode laser and ionization by a dielectric barrier discharge-based probe (laser desorption dielectric barrier discharge ionization mass spectrometry (LD-DBDI-MS)) is presented as an ambient ionization method for the mass spectrometric detection of nonvolatile chemicals on(More)
For the first time, we report a miniaturized approach for isotachophoresis employing the technique of free-flow electrophoresis. Using a micromachined separation chamber with a volume of 200 nL, a sample mixture of fluorescein, eosin G, and acetylsalicylic acid was separated, stacked, and concentrated in less than a minute. Additionally, an isotachophoretic(More)
A multi-dielectric-barrier-nano-electrospray ionization (multi-DB-nESI) emitter setup is presented where the emitters are quasi-simultaneously switched to ignite the respective spray solving the problem of coulombic interferences. Since the switching is done electronically, the sprays can be synchronized to the mass spectrometry (MS) ion trap and the(More)
The mechanism which leads to a dielectric barrier electrospray (DB-ES) was investigated by measurements of the electrical current. It comprises several components, namely, the displacement current, currents which are initiated by electromagnetic transmission, an ion current inside the capillary, which is responsible for the built-up of the potential at the(More)
The parameters influencing the combination of the dielectric barrier electrospray (DB-ES) with an ion trap mass spectrometer are investigated. Two approaches are presented: the application of different polarity cycles in the DB electrospray high voltage signal and the triggering of it to an output signal received by the mass spectrometer. Both approaches(More)
The mechanism of the previously published technique of dielectric barrier electrospray ionization (DB-ESI) was investigated in more detail. Two independent current signals occurring during the DB-ESI could be explained and allocated to sub-processes. The modulated shape of the HV signal, the applied frequency as well as the inner diameter of the emitter(More)
A non-conductive piezo ceramic plate has been used to induce an electric field to generate an electrospray as ionization method for mass spectrometric determination. This technique decreases the risk of undesired discharges, induced by high electric currents. The applicability of the technique is demonstrated and compared with a commercial electrospray for(More)
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