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Rhodobacter sphaeroides Uses a Reductive Route via Propionyl Coenzyme A To Assimilate 3-Hydroxypropionate
It is established that acetyl-CoA is not the exclusive entry point for 3-Hydroxypropionate into the central carbon metabolism and that the reductive conversion of 3-hydroxyPropionate to propionyl- coenzyme A is a necessary route for the assimilation of this molecule by R. sphaeroides. Expand
Transcriptional Regulation by the Short-Chain Fatty Acyl Coenzyme A Regulator (ScfR) PccR Controls Propionyl Coenzyme A Assimilation by Rhodobacter sphaeroides
This work identifies a regulator (PccR) that prevents the accumulation of propionyl-CoA by controlling expression of the gene encoding propiony- coenzyme A carboxylase, which is responsible for proponyl- CoA consumption by Rhodobacter sphaeroides. Expand
Assignment of function to a domain of unknown function: DUF1537 is a new kinase family in catabolic pathways for acid sugars
This study combined high-throughput ligand screening results for transport system solute binding proteins with the synergetic analysis of sequence similarity networks and genome neighborhood networks to establish that the members of the DUF1537 family are novel ATP-dependent four-carbon sugar kinases. Expand
A General Strategy for the Discovery of Metabolic Pathways: d-Threitol, l-Threitol, and Erythritol Utilization in Mycobacterium smegmatis.
We describe a general integrated bioinformatic and experimental strategy to discover the in vitro enzymatic activities and in vivo functions (metabolic pathways) of uncharacterized enzymes discoveredExpand
Functional assignment of multiple catabolic pathways for d-apiose
A bioinformatic strategy beginning with solute-binding proteins involved in sugar transport led to the functional annotation of four previously unknown catabolic pathways of the branched pentose d-apiose. Expand
Characterization of an L-Ascorbate Catabolic Pathway with Unprecedented Enzymatic Transformations.
RNAseq identified eight candidate catabolic genes, sequence similarity networks and genome neighborhood networks guided predictions for function of the encoded proteins, and the predictions were confirmed by in vitro assays and in vivo growth phenotypes of gene dele-tion mutants. Expand
Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine.
Avi_5305, an Agrobacterium vitis SBP belonging to Pfam13407, was screened by differential scanning fluorimetry (DSF) and found to be stabilized by D-glucosamine and D-galactosamine. Expand
Correction to "A General Strategy for the Discovery of Metabolic Pathways: D-Threitol, L-Threitol, and Erythritol Utilization in Mycobacterium smegmatis".
A revised Figure 1 that shows the correct catabolic pathway for L-threitol is presented here and the reported functions for EltD and DerI2 are correct, so the substrate and product of the pathway are unchanged. Expand
An Investigation into Carbon Flow through the Metabolic Networks of Rhodobacter sphaeroides
This book is intended to serve as a “roadmap” for future generations of writers to consider the work they wish to write. Expand