Michael P. Hughes

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In innate immunity, microbial components stimulate macrophages to produce antimicrobial substances, cytokines, other proinflammatory mediators, and IFNs via TLRs, which trigger signaling pathways activating NF-kappaB, MAPKs, and IFN response factors. We show in this study that, in contrast to its activating role in T cells, in macrophages the protein(More)
Submicron particles such as latex spheres and viruses can be manipulated and characterized using dielectrophoresis. By the use of appropriate microelectrode arrays, particles can be trapped or moved between regions of high or low electric fields. The magnitude and direction of the dielectrophoretic force on the particle depends on its dielectric properties,(More)
Dielectrophoresis--the induced motion of polarizable particles in nonuniform fields--has proven to be an effective method for the separation of bioparticles such as cells, viruses and proteins. In this paper, the application of dielectrophoresis in miniaturized laboratories-on-a-chip is discussed, and strategies are described for using dielectrophoresis(More)
The stable negative dielectrophoretic trapping of single sub-micrometre bioparticles is demonstrated. Single virus particles, capsids and latex spheres, with diameters in the range 93–250 nm, have been trapped. An estimate for the minimum size of particle that may be stably trapped against Brownian motion is made and the trapping characteristics used to(More)
BACKGROUND Calcium channel blockers are the most commonly prescribed drugs for people with primary Raynaud's phenomenon. Primary Raynaud's phenomenon is a common condition characterised by an exaggerated vasospastic response to cold or emotion: classically the digits (fingers and toes) turn white, then blue, then red. This is an update of the review first(More)
Myositis ossificans is an uncommon disorder, which is commonly mistaken for a malignant muscle disease before histologic examination. Trauma is the most common cause of the acquired form of the disease; however, atraumatic cases have been described. The factors responsible for extraosseous ossification, such as the role of bone morphogenic protein 4, are(More)
We have used dielectrophoresis to determine the dielectric properties of human chronic myelogeneous leukaemic (K562) cells during apoptosis (programmed cell death). Our results indicate that K562 cells increase markedly in cytoplasmic conductivity from 0.28 S/m to 0.50 S/m within the first 4 h following treatment with staurosporine, which then lasts beyond(More)
Multilayering microelectrode techniques have been developed to construct large-area travelling-wave dielectrophoresis (TWD) separators. Novel electrode designs have enabled arrays with up to 25 000 electrodes to be constructed. The separation capacity of a 1000 electrode (20 mm) array has been demonstrated by separating components of whole blood. The(More)
In silico toxicology prediction is an extremely challenging area because many toxicological effects are a result of changes in multiple physiological processes. In this article we discuss limitations and strengths of these in silico tools. Additionally, we look at different parameters that are necessary to make the best use of these tools, and also how to(More)