• Publications
  • Influence
Metabolic engineering of Saccharomyces cerevisiae for de novo production of dihydrochalcones with known antioxidant, antidiabetic, and sweet tasting properties
TLDR
De novo production of phloretin, the first committed dihydrochalcone, was achieved by co-expression of additional relevant pathway enzymes by exploiting substrate flexibility of enzymes involved in flavonoid biosynthesis. Expand
Yeast artificial chromosomes employed for random assembly of biosynthetic pathways and production of diverse compounds in Saccharomyces cerevisiae
TLDR
A novel strategy for production of diverse natural products, comprising the expression of an unprecedented large number of biosynthetic genes in a heterologous host is described, particularly suited for generating high numbers of structurally diverse compounds. Expand
Improving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double-bond reductase
TLDR
Phenylpropanoids, such as flavonoids and stilbenoids, are of great commercial interest, and their production in Saccharomyces cerevisiae is a very promising strategy, but each step of the process must be optimised. Expand
De novo biosynthesis of anthocyanins in Saccharomyces cerevisiae
TLDR
De novo biosynthesis of ACNs was shown for the first time in yeast and for theFirst time in a single microorganism and represents an important step towards sustainable industrial production of these highly relevant molecules in yeast. Expand
Discovery and Reconstitution of the Cycloclavine Biosynthetic Pathway—Enzymatic Formation of a Cyclopropyl Group†
TLDR
The discovery of the biosynthetic pathway of cycloclavine, a complex ergot alkaloid containing a cyclopropyl moiety, is reported, demonstrating the feasibility of the heterologous expression of these complex alkaloids. Expand
Reconstruction of the biosynthetic pathway for the core fungal polyketide scaffold rubrofusarin in Saccharomyces cerevisiae
TLDR
The reconstructed pathway for rubrofusarin in S. cerevisiae allows the production of a core scaffold molecule with a branch-point role in several fungal polyketide pathways, thus paving the way for production of further natural pigments and bioactive molecules. Expand
The important ergot alkaloid intermediate chanoclavine-I produced in the yeast Saccharomyces cerevisiae by the combined action of EasC and EasE from Aspergillus japonicus
TLDR
It is shown here that EasE and EasC are both necessary and sufficient for the production of chanoclavine-I in yeast, and important new information is provided about the involvement of ER and protein folding for proper functional expression of EasE. Expand
Indirect and direct routes to C-glycosylated flavones in Saccharomyces cerevisiae
TLDR
Yeast is featured as a promising host for production of flavone C-glycosides, and it provides a set of tools and strains for identifying and studying CGTs and their mechanisms of C- glycosylation. Expand
Oestrogenic potencies of Zeranol, oestradiol, diethylstilboestrol, Bisphenol-A and genistein: implications for exposure assessment of potential endocrine disrupters
TLDR
The very high potency of Zeranol compared with other potential endocrine disrupters suggests that Zeranol intake from beef products could have greater impact on consumers than the amounts of the known or suspected endocrinedisrupters that have been found in food. Expand
Discovery and Reconstitution of the Cycloclavine Biosynthetic Pathway—Enzymatic Formation of a Cyclopropyl Group**
TLDR
The discovery of the biosynthetic pathway of cycloclavine, a complex ergot alkaloid containing a cyclopropyl moiety, is reported, demonstrating the feasibility of the heterologous expression of these complex alkaloids. Expand
...
1
2
...