Michael John Miller

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BACKGROUND Individuals with inactive alleles of the fucosyltransferase 2 gene (FUT2; termed the 'secretor' gene) are common in many populations. Some members of the genus Bifidobacterium, common infant gut commensals, are known to consume 2'-fucosylated glycans found in the breast milk of secretor mothers. We investigated the effects of maternal secretor(More)
Microarray analysis of the genome of Lactobacillus acidophilus identified a number of operons that were differentially expressed in response to carbohydrate source or constitutively expressed regardless of carbohydrate source. These included operons implicated in the transport and catabolism of fructooligosaccharides (FOS), lactose (lac), trehalose (tre)(More)
BACKGROUND Lactobacilli can utilize a variety of carbohydrates which reflects the nutrient availability in their respective environments. A common lactobacilli in the human gastrointestinal tract, Lactobacillus gasseri, was selected for further study. The currently available annotation of the L. gasseri ATCC 33323 genome describes numerous putative genes(More)
BACKGROUND 2'-Fucosyllactose (2-FL) is a functional oligosaccharide present in human milk which protects against the infection of enteric pathogens. Because 2-FL can be synthesized through the enzymatic fucosylation of lactose with guanosine 5'-diphosphate (GDP)-l-fucose by α-1,2-fucosyltransferase (FucT2), an 2-FL producing Escherichia coli can be(More)
Brassicales contain a myrosinase enzyme that hydrolyzes glucosinolates to form toxic isothiocyanates (ITC), as a defense against bacteria, fungi, insects and herbivores including man. Low levels of ITC trigger a host defense system in mammals that protects them against chronic diseases. Because humans typically cook their brassica vegetables, destroying(More)
Recently, prebiotic supplementation of infant formula has become common practice; however the impact on the intestinal microbiota has not been completely elucidated. In this study, neonatal piglets were randomized to: formula (FORM, n = 8), formula supplemented with 2 g/L each galactooligosaccharides (GOS) and polydextrose (PDX, F+GP, n = 9) or a sow-reared(More)
Let S(n) be the set of all polynomials of degree n with all roots in the unit disk, and define d(P) to be the maximum of the distances from each of the roots of a polynomial P to that root's nearest critical point. In this notation, Sendov's conjecture asserts that d(P) ≤ 1 for every P ∈ S(n). Define P ∈ S(n) to be locally extremal if d(P) ≥ d(Q) for all(More)
Define S(n, β) to be the set of complex polynomials of degree n ≥ 2 with all roots in the unit disk and at least one root at β. For a polynomial P , define |P | β to be the distance between β and the closest root of the derivative P ′. Finally, define rn(β) = sup{|P | β : P ∈ S(n, β)}. In this notation, a conjecture of Bl. Sendov claims that rn(β) ≤ 1. In(More)