Michael J. Hartenstine

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Repetitions of CAG or CTG triplets in DNA can form intrastrand hairpin loops with combinations of normal and mismatched base pairs that easily rearrange. Such loops may promote primer-template slippage in DNA replication or repair to give triplet-repeat expansions like those associated with neurodegenerative diseases. Using self-priming sequences (e.g.(More)
Mammalian phosphoinositide-specific phospholipase C (PLC) (EC: 3.1.4.11) catalyze hydrolysis of the phosphodiester linking the inositol polar head group to diacylglycerol (DAG). PI(4)P and PI(4,5)P2 are the preferred substrates; whereas 3-phosphorylated phosphoinositides are not accommodated. There are at least six known types of mammalian PLC: b1–4, g1–2,(More)
Lengthy expansions of trinucleotide repeats are found in DNA of patients suffering severe neurodegenerative age-related diseases. Using a synthetic self-priming DNA, containing CAG and CTG repeats implicated in Huntington's disease and several other neurological disorders, we measure the equilibrium distribution of hairpin folding and generate triplet(More)
Using synthetic DNA constructs in vitro, we find that human DNA polymerase beta effectively catalyzes CAG/CTG triplet repeat expansions by slippage initiated at nicks or 1-base gaps within short (14 triplet) repeat tracts in DNA duplexes under physiological conditions. In the same constructs, Escherichia coli DNA polymerase I Klenow Fragment exo(-) is much(More)
A protein carboxyl methyltransferase activity (PCMT) with a specificity for age-damaged protein D-aspartyl and L-isoaspartyl residues (E.C. 2.1.1.77) has been identified and cloned in Drosophila. The Drosophila gene was localized by chromosome in-situ hybridization to region 83AB of the third chromosome. The methyltransferase coding sequence is distributed(More)
A polyethylene glycol (PEG) polymer was synthesized using a dip-coating procedure on 316L stainless-steel (SS) substrate precoated with a primer that consisted of radiofrequency inductively coupled plasma-polymerized di (ethylene glycol) vinyl ether (EO2V). The primer and PEG composite film was studied with profilometer, optical microscope, scanning(More)
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