Michael J Eddy

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We have discovered that DNA supercoiling, in the absence of replication proteins, induces localized unwinding in the Escherichia coli replication origin (oriC) at the same sequence opened by the dnaA initiator protein. The DNA helix at the tandemly repeated, 13mer sequence is thermodynamically unstable, as evidenced by hypersensitivity to(More)
A long A+T-rich sequence in supercoiled pBR322 DNA is hypersensitive to single-strand-specific nucleases at 37 degrees C but not at reduced temperature. The basis for the nuclease hypersensitivity is stable DNA unwinding as revealed by (i) the same temperature dependence for hypersensitivity and for stable unwinding of plasmid topoisomers after(More)
The accurate evaluation of cardiovascular reactions to psychological challenge requires stable baselines against which change can be evaluated. When more than one challenge is employed, the recovery of this baseline becomes important in order to avoid carryover effects. Resting periods, even those of 20 min or more, do not guarantee baseline stability. We(More)
This paper describes efforts to reduce measurement error in the assessment of cardiovascular reactivity by standardizing task requirements and by aggregating data across tasks and testing sessions. Using these methods, reliable measures of reactivity (.80 or greater) were obtained on five different measures of cardiovascular function (heart rate, systolic(More)
Researchers are interested in respiratory sinus arrhythmia (RSA) as an index of cardiac vagal activity. Yet, debate exists about how to account for respiratory influences on quantitative indices of RSA. T. Ritz, M. Thons, and B. Dahme (2001) developed a within-individual correction procedure by which the effects of respiration on RSA may be estimated using(More)
Recent evidence has suggested a possible relationship between the tendency to exhibit excessive cardiovascular reactions during psychological challenge and the risk of cardiovascular disease. Valid techniques for reliably assessing such reactions are, however, minimally available. A test battery for the assessment of cardiovascular reactivity to(More)
This paper studies the socioeconomic disparities in self-perceived oral-health among Chilean adults. Data are from an in-depth survey that includes detailed questions on oral health, dental care, and socioeconomic status for a sample of people in Santiago, Chile in 2011, complemented with nationally representative surveys to check for selection bias. Oral(More)
Social impact bonds (SIBs) have the potential to improve the efficiency of government health-care spending in South-East Asia. In a SIB, governments sign a pay-for-performance contract with one or several providers of health-care services, and the providers borrow up-front capital from investors. Governments outside South-East Asia have started to(More)
This replication study was designed to examine the reliability of individual differences in cardiovascular responsivity to a standardized PC-based cognitive task protocol (Kamarck et al., 1992) in a female community sample. Thirty women, both black and white (ages 25-44 years), were administered the protocol twice with a 1-month retest interval using a(More)