Michael J. Donovan

Learn More
Aptamers are single-stranded synthetic DNA- or RNA-based oligonucleotides that fold into various shapes to bind to a specific target, which includes proteins, metals, and molecules. Aptamers have high affinity and high specificity that are comparable to that of antibodies. They are obtained using iterative method, called (Systematic Evolution of Ligands by(More)
We present a new system for automated localization and quantification of the expression of protein biomarkers in immunofluorescence (IF) microscopic images. The system includes a novel method for discriminating the biomarker signal from background, where signal may be the expression of any of the many biomarkers or counterstains used in IF. The method is(More)
Personalized therapy provides the best outcome of cancer care and its implementation in the clinic has been greatly facilitated by recent convergence of enormous progress in basic cancer research, rapid advancement of new tumor profiling technologies, and an expanding compendium of targeted cancer therapeutics. We developed a personalized cancer therapy(More)
  • Piruz Motamedinia, Anna N. Scott, Kendall L. Bate, Neda Sadeghi, Guillermo Salazar, Edan Shapiro +11 others
  • 2016
PURPOSE The analysis of exosome/microvesicle (extracellular vesicles (EVs)) and the RNA packaged within them (exoRNA) has the potential to provide a non-invasive platform to detect and monitor disease related gene expression potentially in lieu of more invasive procedures such as biopsy. However, few studies have tested the diagnostic potential of EV(More)
Morphometric features characterizing the fusion and fragmentation of the glandular architecture of advanced prostate cancer have not previously been based upon the automated segmentation of discrete gland rings, due in part to the difficulty of extracting these structures from the H&E stained tissues. We present a novel approach for segmenting gland rings(More)
  • 1