Michael J Bocek

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The hormone estrogen (E2) binds the estrogen receptor to promote transcription of E2-responsive genes in the breast and other tissues. E2 also has links to genomic instability, and elevated E2 levels are tied to breast cancer. Here, we show that E2 stimulation causes a rapid, global increase in the formation of R-loops, co-transcriptional RNA-DNA products,(More)
In the developing pancreas of human embryos and fetusses (in weeks 4 to 20 of the intrauterine life) activities of aminopeptidase A (APA) and M (APM), dipeptidyl peptidase IV (DPP IV), and gamma-glutamyl transferase (GGT) were studied in cryostat sections using methods as recommended by Lojda (J. Histochem. Cytochem. 29, 481 (1981)). In the youngest embryos(More)
Targeted gene delivery vectors can enhance cellular specificity and transfection efficiency. We demonstrated previously that conjugation of Tet1, a peptide that binds to the GT1b ganglioside, to polyethylenimine results in preferential transfection of neural progenitor cells in vivo. In this work, we investigate the effect of Tet1 ligand density on gene(More)
The expression of PCNA was proved in some organs of human fetuses aged from 10 to 25 weeks of the intrauterine development. The distinct expression of PCNA was demonstrated in the nuclei of the myoblasts of the ventricles and atria of the fetal heart. The high expression of PCNA was found in the nuclei of the epithelial cells of the fetal bronchial tree,(More)
Several cationic antimicrobial peptides have been investigated as potential anti-cancer drugs due to their demonstrated selective toxicity towards cancer cells relative to normal cells. For example, intracellular delivery of KLA, a pro-apoptotic peptide, results in toxicity against a variety of cancer cell lines; however, the relatively low activity and(More)
Activities of the following enzymes were assessed in cryostat sections of human embryonic and fetal placentae aged 7 to 22 weeks of the intrauterine life using the standard methods recommended by Lojda et al. (1978): alkaline phosphatase (AIP), and acid phosphatase (AcP), non-specific esterase (ANE), ATP-cleaving enzymes (ATP-ase), beta-glucuronidase,(More)
Conflicts between transcription and replication are a potent source of DNA damage. Co-transcriptional R-loops could aggravate such conflicts by creating an additional barrier to replication fork progression. Here, we use a defined episomal system to investigate how conflict orientation and R-loop formation influence genome stability in human cells. R-loops,(More)
The activities of some enzymes (phosphatases, esterases peptidases, and dehydrogenases) were studied. The activities of the enzymes in the youngest embryos (4-8 weeks) were relatively low (ALP-activity on membranes of the primitive capillary endothelium, ACP and UE activities in the cytoplasm of the epithelial reticulum cells). The DPP IV activity was(More)