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A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
TLDR
This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation
TLDR
To compare the architectures and domain organization of diverse Cas9 proteins, the atomic structures of Cas9 from Streptococcus pyogenes and Actinomyces naeslundii and AnaCas9 were determined by x-ray crystallography and three-dimensional reconstructions of apo-SpyCas9, SpyCas9:RNA, and SpyCas 9:RNA:DNA were obtained by negative-stain single-particle electron microscopy.
Histone degradation in response to DNA damage enhances chromatin dynamics and recombination rates
TLDR
It is proposed that a generalized reduction in nucleosome occupancy is an integral part of the DNA damage response in yeast that provides mechanisms for enhanced chromatin mobility and homology search.
Chromatin and nucleosome dynamics in DNA damage and repair.
TLDR
An overview of the function of chromatin structure and its dynamic changes in response to genotoxic stress is presented, focusing on both subnuclear organization and the physical mobility of DNA.
Nucleosome remodelers in double-strand break repair.
Loss of an H3K9me anchor rescues laminopathy-linked changes in nuclear organization and muscle function in an Emery-Dreifuss muscular dystrophy model.
TLDR
It is suggested that a novel therapeutic pathway for EDMD using the coupling of the LMN-Y59C mutation with the ablation of CEC-4, a chromodomain protein that anchors H3K9-methylated chromatin at the nuclear envelope (NE), suppressed the muscle-associated disease phenotypes.
Chromosome Dynamics in Response to DNA Damage.
TLDR
Key regulators of chromatin motion in physiological conditions or after damage induction are reviewed here.
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