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Three 16S rRNA hybridization probes were developed and tested for genus-specific detection of Bifidobacterium species in the human fecal flora. Variable regions V2, V4, and V8 of the 16S rRNA contained sequences unique to this genus and proved applicable as target sites for oligodeoxynucleotide probes. Determination of the genus specificity of the(More)
A method for automatic identification of diatoms (single-celled algae with silica shells) based on extraction of features on the contour of the cells by multi-scale mathematical morphology is presented. After extracting the contour of the cell, it is smoothed adaptively, encoded using Freeman chain code, and converted into a curvature representation which(More)
In this paper, we describe a multiscale and multishape morphological method for pattern-based analysis and classification of gray-scale images using connected operators. Compared with existing methods, which use structuring elements, our method has three advantages. First, in our method, the time needed for computing pattern spectra does not depend on the(More)
The techniques of push-pull perfusion and radioimmunoassay were used to determine concentrations of arginine vasopressin (AVP) in extracellular fluid derived from the ventral septal area (VSA) of the rat brain following antipyresis elicited by acetaminophen or indomethacin in conscious and unrestrained rats. Reduction of bacterial lipopolysaccharide(More)
A novel, physically motivated deformable model for shape recovery and segmentation is presented. The model, referred to as the charged-particle model (CPM), is inspired by classical electrodynamics and is based on a simulation of charged particles moving in an electrostatic field. The charges are attracted towards the contours of the objects of interest by(More)
Morphological attribute filters have not previously been parallelized, mainly because they are both global and non-separable. We propose a parallel algorithm which achieves efficient parallelism for a large class of attribute filters, including attribute openings, closings, thinnings and thickenings, based on Salembier's Max-Trees and Min-trees. The image(More)
Specific binding of the hydrophilic radioligand [3H]CGP-12177 to cell surface (functional) beta-adrenoreceptors was quantified in ventricular micropunches (2 mm diameter, 350 microns thickness) from seawater-acclimated rainbow trout held at 7-9 degrees. Binding was stereospecific, saturable, of high affinity, and displaceable by appropriate agonists and(More)
An efficient algorithm is presented for the computation of grayscale morphological operations with arbitrary 2D flat structuring elements (S.E.). The required computing time is independent of the image content and of the number of gray levels used. It always outperforms the only existing comparable method, which was proposed in the work by Van Droogenbroeck(More)
Component trees are important data structures for computation of connected attribute filters. Though some of the available algorithms are suitable for high-dynamic range, and in particular floating point data, none are suitable for computation of component trees for so-called second-generation, and mask-based connectivity. The latter allow generalization of(More)
Methylphenidate (Ritalin) is a psychostimulant drug used to treat children with attention deficit hyperactivity disorder. Despite its widespread and increasing clinical use, little is known about the long-term consequences of drug treatment. We compared the effects of a single injection of methylphenidate with that of long-term methylphenidate injections(More)