Michael G. Nanazashvili

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It was shown that the intact rabbit sera interact with chromatin and thymocyte nuclei in a quantitative reaction of complement fixation and immunoluminescence analysis, respectively. Purified immunoglobulins G (IgG) and Fab fragments isolated from them were also found capable of such interactions. The interactions are not caused by Ig aggregate absorption(More)
Rabbit antibodies against rat liver and thymus are obtained. IgG isolated from antithymus sera interact with homologous chromatin but do not interact with chromatin from hepatocytes. Antiliver IgG interact with chromatin from both organs: however, their pretreatment with thymocyte chromatin results in antibodies which interact only with liver chromatin.(More)
Differences in antigenic structure of chromatin from normal and tumour cells were studied using rabbit antibodies against chromatin of the rat liver, Zaidela ascite hepatoma and solid hepatoma 27 cells. It is shown that carcinogenesis is accompanied by loss of a part of normal liver antigens. All the antigens which are not indicated in solid hepatoma were(More)
Ca/Mg-dependent nuclease is a possible key enzyme of apoptosis. We isolated and purified nucleases from the human and rat thymus to a homogeneous state and compared some properties of the obtained preparations with those of the earlier isolated Ca/Mg-dependent nuclease from the calf thymus. The activity of the nuclease from the human thymus in the presence(More)
The accessibility of protein antigenic determinants of rat thymocyte chromatin was studied in a reaction of complement fixation, using antisera from animals immunized with chromatin or non-histone proteins and control sera containing natural antichromatin IgG. It was shown that the bulk of the antigenic determinants of intact chromatin are inaccessible for(More)
Rabbit antibodies against rat thymus and liver chromatin are obtained. Antithymus IgG are found to interact only with homologous chromatin, while antiliver IgG interact with both liver and thymus chromatin. After preincubation of antiliver IgG with thymus chromatin the antibodies interact with homologous chromatin only. Thus chromatin of both organs(More)
The antisera of rabbits immunized with chromatin and non-histone proteins (NHP) were compared to natural antibodies against chromatin. It was shown that in indirect immunofluorescence and complement fixation assay the immune and control sera interact with chromatin at close dilutions. The IgG purified from the immune sera which do not bind to DEAE-cellulose(More)
Localization of malignant cell antigens which are not detected in the liver chromatin was investigated by antibodies to chromatin of Zajdela ascite hepatoma and solid hepatoma 27. Antibodies to chromatin of Zajdela ascite hepatoma do not interact with nuclear matrix of both hepatoma and liver cells. Zajdela ascite hepatoma and solid hepatoma 27 chromatin(More)
Rabbit antibodies against rat thymus and liver chromatin are obtained. Antithymus IgG are found to interact only with homologous chromatin, while antiliver IgG interact with both liver and thymus chromatin. After preincubation of antiliver IgG with thymus chromatin the antibodies interact with homologous chromatin only. Thus chromatin of both organs(More)