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The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based technology to screen 102 bait proteins from Drosophila melanogaster, most of them orthologous to human cancer-related and/or signaling proteins, against(More)
Heart disease is a leading cause of death in newborn children and in adults. Efforts to promote cardiac repair through the use of stem cells hold promise but typically involve isolation and introduction of progenitor cells. Here, we show that the G-actin sequestering peptide thymosin beta4 promotes myocardial and endothelial cell migration in the embryonic(More)
We examined the statistical analyses of experimental data in 95 papers published during 1966-1990 on the ecology, physiology, and behavior of freshwater invertebrate zooplankti-vores. Serious statistical errors were found in 51% of the papers. The frequencies of particular types of errors were as follows: sacrificial pseudoreplication (31%), simple(More)
We have recently demonstrated that macrophage migration inhibitory factor (MIF) is a myocardial depressant protein and that MIF mediates late, prolonged cardiac dysfunction after endotoxin challenge in mice. Because many factors, including endotoxin, have been implicated in the pathogenesis of cardiac dysfunction after burn injury, we tested the hypothesis(More)
The border region of Baja California in Mexico and California in the United States is a biologically diverse and unique landscape that forms a portion of one of the world's global biodiversity hotspots. While the natural resources of this border region are continuous and interconnected, land conservation practices on either side of the international(More)
Atypical protein kinase C (aPKC) isoforms have been implicated in cell polarisation and migration through association with Cdc42 and Par6. In distinct migratory models, the Exocyst complex has been shown to be involved in secretory events and migration. By RNA interference (RNAi) we show that the polarised delivery of the Exocyst to the leading edge of(More)
Macrophage migration inhibitory factor (MIF) is a pluripotent proinflammatory cytokine that is ubiquitously expressed in organs, including the heart. However, no specific role for MIF in modulating cardiac performance has yet been described. Therefore, we examined cardiac MIF expression in mice after LPS challenge (4 mg/kg) and tested the hypothesis that(More)
reprograms calcium signaling and regulates myoglobin expression. an oxygen storage molecule that is selectively expressed in cardiac and slow-twitch skeletal muscles that have a high oxygen demand. Numerous studies have implicated hypoxia in the regulation of myo-globin expression as an adaptive response to hypoxic stress. However, the details of this(More)
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This paper describes an evolutionary algorithm that was developed for catalog design. This algorithm is based on genetic algorithms, but uses an object-oriented coding scheme to represent a design, and introduces unique crossover and mutation operatolw. To account for the dependence of system pe~7Ormance on both system configuration and component selection,(More)