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Here we report that RNA polymerase (pol) III transcription is repressed in response to DNA damage by downregulation of TFIIIB, the core component of the pol III transcriptional machinery. Protein kinase CK2 transduces this stress signal to TFIIIB. CK2 associates with and normally activates the TATA binding protein (TBP) subunit of TFIIIB. The beta(More)
In Tadoma, speech is received by placing a hand on the talker's face and monitoring actions associated with speech production. Our initial research has documented the speech perception, speech production, and linguistic abilities of deaf-blind individuals highly trained in Tadoma. This research has demonstrated that good speech reception can be achieved(More)
Certain deaf-blind persons have been taught, through the Tadoma method of speechreading, to use vibrotactile cues from the face and neck to understand speech. This paper reports the results of preliminary tests of the speechreading ability of one adult Tadoma user. The tests were of four major types: (1) discrimination of speech stimuli; (2) recognition of(More)
Little is known about what enzyme complexes or mechanisms control global lysine acetylation in the amino-terminal tails of the histones. Here, we show that glucose induces overall acetylation of H3 K9, 18, 27 and H4 K5, 8, 12 in quiescent yeast cells mainly by stimulating two KATs, Gcn5 and Esa1. Genetic and pharmacological perturbation of carbon(More)
A simple method for preparing chromatin assembly extracts has not been available for budding yeast. Here I describe such a method in detail. The assembly extract, a crude 100,000g supernatant, is prepared from cells disrupted in a manual or motorized grinder while they are frozen. The core histones and all soluble protein factors required for chromatin(More)
Performance on identifying consonant and vowel stimuli through the Tadoma methods of speechreading was studied for a deaf-blind, experienced user of Tadoma. For a set of 24 consonants in CV syllables, performance averaged 55%. For a set of 15 vowels and diphthongs in CVC syllables, performance averaged 56%. Confusion matrices derived from the identification(More)
In the Tadoma method of communication, deaf-blind individuals receive speech by placing a hand on the face and neck of the talker and monitoring actions associated with speech production. Previous research has documented the speech perception, speech production, and linguistic abilities of highly experienced users of the Tadoma method. The current study was(More)
Staggered Spondaic Word (SSW) test results were studied in 93 normally achieving children and 97 children referred to a learning disabilities clinic. All subjects were 8-11 years of age. The SSW test was found to differentiate between the normally achieving children and those experiencing classroom learning difficulties. Further, it was found that the(More)
The histone chaperone Asf1 and the chromatin remodeler SWI/SNF have been separately implicated in derepression of the DNA damage response (DDR) genes in yeast cells treated with genotoxins that cause replication interference. Using genetic and biochemical approaches, we have tested if derepression of the DDR genes in budding yeast involves functional(More)
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