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Procedures are described for the isolation of lipoproteins from human serum by precipitation with polyanions and divalent cations. A mixture of low and very low density lipoproteins can be prepared without ultracentrifugation by precipitation with heparin and either MnCl(2) alone or MgCl(2) plus sucrose. In both cases the precipitation is reversible,(More)
We present a new approach to the automatic layout design for VLSI chips which incorporates timing information to influence the placement and wiring processes. This approach is an extension of the hierarchical layout method, in which placement and wiring are performed simultaneously [1]. We add a third phase of timing to the hierarchy, without affecting the(More)
We propose a new approach to automatic wire routing of VLSI chips which is applicable to interconnection problem in uniform structures such as gate arrays, switchboxes, channels. Popularity of gate arrays technologies still remains high among VLSI chip manufacturers and, as the scale of integration grows, the interconnection problem becomes increasingly(More)
The human MUC1 gene expresses at least 2 type 1 membrane proteins: MUC1/REP, a polymorphic high m.w. MUC1 glycoprotein often highly expressed in breast cancer tissues and containing a variable number of tandem 20 amino acid repeat units, and the MUC1/Y protein, which lacks this repeat array and, therefore, is not polymorphic. Despite their documented(More)
Studies in recent years have suggested that measurement of high density lipoprotein (HDL) subclasses may provide significant information beyond that provided by measurement of total HDL. However, conventional methodology for separation of HDL subclasses involves various types of ultracentrifugation that are time-consuming, costly, and not suitable for many(More)
The channel routing problem is a special case of the wire routing problem when interconnections have to be performed within a rectangular strip having no obstructions, between terminals located on opposite sides of the rectangle. We present here a new channel routing algorithm, based on reduction of the problem to the case of a (2&#215;<italic>n</italic>)(More)
We describe a rapid and reliable three-step precipitation procedure for the isolation of large amounts of the two major components of high density lipoproteins (HDL) in human serum. Precipitation was accomplished by means of dextran sulfate (DS) of mol. wt. 500,000 and MgCl2. First, all apoB-associated lipoproteins of any density were selectively(More)