Michael Burroughs

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Using a monoclonal antibody to chondroitin 6-sulfate, a major constituent of the cone matrix sheath, the location of the cone matrix sheath and the relative distribution of interphotoreceptor retinoid-binding protein (IRBP) was examined by light and electron microscopic immunocytochemistry. Chondroitin 6-sulfate immunoreactivity was localized to regions(More)
The distribution of IRBP was examined in postnatal developing retinas of rds (020/A) mutant mice and Balb/c controls by EM immunocytochemistry. Light labeling for IRBP was detected in mutant and control retinas by postnatal day 9 (P9) largely in the interphotoreceptor matrix (IPM). At P14, some photoreceptors in the rds retina showed a higher density of(More)
Light microscopic immunogold cytochemistry was used to examine the distribution of interphotoreceptor retinoid-binding protein (IRBP) in the interphotoreceptor matrix (IPM) surrounding rod and cone photoreceptors. Silver enhancement of retinas reacted with anti-IRBP antibodies using the two stage labeling procedure showed dense staining of the IPM around(More)
Heterotrimeric guanine nucleotide-binding proteins (G proteins) composed of three subunits α, β, γ mediate activation of multiple intracellular signaling cascades initiated by G protein-coupled receptors (GPCRs). Previously our laboratory identified small molecules that bind to Gβγ and interfere with or enhance binding of select effectors with Gβγ. To(More)
G-protein betagamma (Gbetagamma) subunits interact with a wide range of molecular partners including: G(alpha) subunits, effectors, peptides, and small molecule inhibitors. The molecular mechanisms underlying the ability to accommodate this wide range of structurally distinct binding partners are not well understood. To uncover the role of protein(More)
The presence of interphotoreceptor retinoid-binding protein (IRBP) in the Golgi apparatus of monkey cone photoreceptors was examined by electron microscopic immunocytochemistry. Intracellular labeling for IRBP was light to moderate over the Golgi complex of foveal and peripheral cones in the retinas of all four monkeys examined. Composite drawings of two to(More)
Phospholipase Cβ (PLCβ) enzymes are activated by G protein-coupled receptors through receptor-catalyzed guanine nucleotide exchange on Gαβγ heterotrimers containing Gq family G proteins. Here we report evidence for a direct interaction between M3 muscarinic receptor (M3R) and PLCβ3. Both expressed and endogenous M3R interacted with PLCβ in(More)
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