Michael B. Stephenson

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We have developed a rapid procedure for isolating a fraction enriched in plasma membrane from Dunaliella salina using an aqueous two-phase system (dextran/polyethylene glycol, 6.7%/6.7%). An enriched plasma membrane fraction, free of chloroplast and mitochondrial contamination, could be obtained in 2.5 hours. Plasma membrane proteins, which accounted for(More)
The unicellular, wall-less alga Dunaliella salina has been shown to contain an array of proteins modified by the covalent attachment of fatty acids. Myristic acid (14:0) comprised approximately 80% by weight of the protein-linked acyl groups in samples derived from cells cultured in medium containing 1.7 molar NaCl and 93% in samples from cells grown in(More)
This paper describes an algorithm for clipping and capping polyhedrons and a display system for three-dimensional finite element math models to facilitate viewing internal surfaces in the form of continuous tone computer generated color images. A plane is passed through the model with the result that the elements (or portions thereof) on one side of the(More)
The design of a set of benchmarks for evaluating the capabilities of high-performance engineering workstations is described. The benchmarks were designed to evaluate the workstations' capacity to calculate and display solid finite-element or finite-difference data. After the benchmarks were run on several workstations, a cost performance analysis was(More)
An algorithm is presented for rendering scalar field data which reduces rendering times by as much as two orders of magnitude over traditional full resolution images. Less than full resolution sampling of the scalar field is performed using a fast ray tracing method. The sampling grid points are output as a set of screen based Gouraud shaded polygons which(More)
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