Michael A. Fisher

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The microbial conversion of sugars to fuels is a promising technology, but the byproducts of biomass pretreatment processes and the fuels themselves are often toxic at industrially relevant levels. One promising solution to these problems is to engineer efflux pumps to secrete fuels and inhibitory chemicals from the cell, increasing microbial tolerance and(More)
Recent investigations using the Monterey Bay Aquarium Research Institutes (MBARI) Remotely Operated Vehicles (ROVs) “Ventana” and “Tiburon” and interpretation of MBARI’s EM 300 30 kHz multibeam bathymetric data show that the northern flank of the Santa Barbara Basin has experienced massive slope failures. Of particular concern is the large (130 km2) Goleta(More)
The Seattle fault, a large, seismically active, east–west-striking fault zone under Seattle, is the best-studied fault within the tectonically active Puget Lowland in western Washington, yet its subsurface geometry and evolution are not well constrained. We combine several analysis and modeling approaches to study the fault geometry and evolution, including(More)
The Oak Ridge fault is a large-offset, south-dipping reverse fault that forms the south boundary of the Ventura Basin in southern California. Previous research indicates that the Oak Ridge fault south of the town of Ventura has been inactive since 200–400 ka ago and that the fault tip is buried by 1 km of Quaternary sediment. However, very high-resolution(More)
The concentration and nature of the electrolyte are key factors determining (1) the equilibrium extent of binding of oligocations or proteins to DNA, (2) the distribution of bound protein between specific and nonspecific sites, and (3) the kinetics of association and dissociation of both specific and nonspecific complexes. Salt concentration may therefore(More)
The prephenate dehydrogenase activity of the bifunctional enzyme chorismate mutase-prephenate dehydrogenase from Escherichia coli catalyzes the oxidative decarboxylation of both prephenate and deoxoprephenate, which lacks the keto group in the side chain (V 78% and V/K 18% those of prephenate). Hydride transfer is to the B side of NAD, and the(More)
Enzymes are indispensable in the effort to produce chemicals from fuels to pharmaceuticals in an ecologically friendly manner. They have the potential to catalyze reactions with high specificity and efficiency without the use of hazardous chemicals. Nature provides an extensive collection of enzymes, but often these must be altered to perform desired(More)
This study examined changes in pH and extractable nutrients in soilsfollowing wetland creation. Sample plots were established in two areas: (1) an old-field with parts that were flooded during wetland creation, and (2) a native wetland in a floodplain of the Ohio River called Green Bottom Swamp. Soils were sampled before inundation and eight months(More)
We interpret seismic-reflection data, which were collected in Santa Monica Bay using a 70-in generator-injector air gun, to show the geologic structure of the continental shelf and slope and of the deep-water, Santa Monica and San Pedro Basins. The goal of this research is to investigate the earthquake hazard posed to urban areas by offshore faults. These(More)
Primary intrinsic deuterium and 13C isotope effects have been determined for liver (LADH) and yeast (YADH) alcohol dehydrogenases with benzyl alcohol as substrate and for yeast aldehyde dehydrogenase (ALDH) with benzaldehyde as substrate. These values have also been determined for LADH as a function of changing nucleotide substrate. As the redox potential(More)