Michael A. Farris

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Four new antibiotic-resistant derivatives of the broad-host-range (bhr) cloning vector pBBR1MCS have been constructed. These new plasmids have several advantages over many of the currently available bhr vectors in that: (i) they are relatively small (< 5.3 kb), (ii) they possess an extended multiple cloning site (MCS), (iii) they allow direct selection of(More)
We tested the hypothesis that plant loss to insects, and thus the relative fitness of an annual, was inversely related to spatial variation in the concentration of its characteristic secondary compound, methylglucosinolate, a mustard oil precursor. We found that methylyglucosinolate concentrations decreased significantly and linearly from the dry to the wet(More)
Monoclonal antibodies (McAb) specific for human IgA, the IgA1 and IgA2 subclasses and the iso-allotypic epitope nA2m(2) have been produced. Three distinct McAb recognize an IgA1-specific epitope expressed in the C alpha 2 domain or the hinge region whilst a further McAb is directed possibly to an IgA1 hinge region epitope. The McAb having nA2m(2)(More)
Secretory Component (SC) was measured in serum samples from patients with a variety of disorders using enzyme-linked immunosorbent assay (ELISA). The overall precision of the method at high and low concentrations of SC was 11% (CV). Serum concentrations of SC were significantly higher in patients with several forms of liver disease (means = 82.37 mg/l +/-(More)
The induction of IgA synthesis in normal peripheral blood lymphocytes cultured in the presence of 5 different B-cell activators was studied by immunofluorescence using monoclonal antibodies to the two subclasses. The surface phenotype of normal cells after overnight culture in the absence of mitogen showed a mean ratio IgA1:IgA2 of 2.7:1; cells with(More)
I investigated the extent and adaptive importance of genetically-based variation in plant water relations in two populations of the annual plant Cleome serrulata found growing along relatively short (<30 m) and mild soil moisture gradients. Field measurements of predawn plant water potentials showed that plants at the dry end of the moisture gradients had(More)
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