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Multiple 18S rDNA sequences were obtained from two single-oocyst-derived lines of each of Eimeria meleagrimitis and Eimeria adenoeides. After analysing the 15 new 18S rDNA sequences from two lines of E. meleagrimitis and 17 new sequences from two lines of E. adenoeides, there were clear indications that divergent, paralogous 18S rDNA copies existed within(More)
For the purpose of re-describing the Eimeria species that infect the turkey (Meleagris gallopavo) and to establish benchmark biological information linked to genetic markers for each species, a strain of Eimeria meleagrimitis Tyzzer 1929 was obtained from a litter sample from a turkey farm in Minnesota, USA in 2008. Multiple pure lines were derived by(More)
Unlike with Eimeria species infecting chickens, specific identification and nomenclature of Eimeria species infecting turkeys is complicated, and in the absence of molecular data, imprecise. In an attempt to reconcile contradictory data reported on oocyst morphometrics and biological descriptions of various Eimeria species infecting turkey, we established(More)
Percutaneous renal biopsy was done in 25 Saudi children whose nephrotic syndrome did not respond to prednisolone. The histologic diagnosis was mesangial proliferative glomerulonephritis in 11 cases; an additional two biopsies had features of both mesangial proliferative glomerulonephritis and focal segmental glomerulosclerosis. Immunofluorescence was(More)
Egg propagated gametocytes of Eimeria tenella (local isolates) were used to prepare the adjuvanted (Amphigen) and nonadjuvanted vaccine(s) and evaluated on the basis of cellular, humoral, and challenge responses. Modified splenic cell migration inhibition test and enzyme-linked immunosorbant assay were used to assess the cellular and humoral responses,(More)
The present paper describes the field evaluation of local gametocyte vaccine and its comparative efficacy with commercial anticoccidial vaccine, LivaCox®, used in breeder and broiler flocks in Pakistan. Humoral immune response in vaccinated and control chickens was monitored by enzyme-linked immunosorbent assay. Results demonstrated significantly elevated(More)
Toxoplasmosis, an infection caused by Toxoplasma (T.) gondii Apicomplexa protozoan, is widespread in humans and other animal species, having already been reported in many countries and different climates. In Pakistan, no data is available on this aspect among food animals. This study was undertaken to determine the seroprevalence of T. gondii infection in(More)
The present paper reports the ability of egg-propagated gametocyte vaccine to induce protective immunity against Eimeria tenella infections in offspring chicks. For this purpose, breeding hens were immunized with adjuvanted gametocyte vaccine orally, being the best vaccine in our previous studies. Eggs collected 2 weeks after the boosting dose were allowed(More)