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The effects of 1:1 flavonoid-Cu(2+) complexes of four flavonoids with different C-ring substituents, quercetin (QU), luteolin (LU), taxifolin (TA), and (+)-catechin (CA), on bovine serum albumin (BSA) were investigated and compared with corresponding free flavonoids by spectroscopic analysis in an attempt to characterize the chemical association taking(More)
Novel magnetic multi-walled carbon nanotubes@Fe(3)O(4) molecularly imprinted polymers (MWNTs@Fe(3)O(4)-MIPs) intended for bovine serum albumin (BSA) recognition were successfully developed. The MWNTs@Fe(3)O(4)-MIPs were characterized with scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). Scanning electron microscopy(More)
Tyrosinase inhibitors play an important role in cosmetic products, food supplements and medicinal industries. In this study, a new facile method based on tyrosinase immobilized magnetic fishing (IMF) coupled with high performance liquid chromatography-diode array detector-tandem mass spectrometry (HPLC-DAD-MS/MS) was developed to screen and identify(More)
Phosphodiesterase (PDE) inhibitors are widely used because of their various pharmacological properties, and natural products are considered the most productive source of PDE inhibitors. In this work, a new ultrafiltration-high-performance liquid chromatography (HPLC)-diode-array detection-mass spectrometry based ligand screening was developed for the first(More)
The interaction between ilaprazole and bovine serum albumin (BSA) has been investigated in the absence and presence of four popular flavonoids with different C-ring structures, quercetin, luteolin, taxifolin, and (+)-catechin, by means of fluorescence spectroscopy. The results indicated that ilaprazole had a strong ability to quench the intrinsic(More)
The aqueous extract of Eucommia ulmoides leaves has been commonly known as Du-zhong tea as a functional health food for the treatment of hypertension, hypercholesterolemia, and fatty liver. This study developed a centrifugal ultrafiltration-high-performance liquid chromatography (HPLC) method for screening and identification of bioactive compounds in E.(More)
The effect of heavy metal ions, Cd(2+), Hg(2+) and Pb(2+) on taxifolin binding to bovine serum albumin (BSA) has been investigated by spectroscopic methods. The results indicated that the presence of heavy metal ions significantly affected the binding modes and binding affinities of taxifolin to BSA, and the effects depended on the type of heavy metal ions.(More)
The interaction of pantoprazole to bovine serum albumin (BSA) has been investigated with and without four popular 5,7,3',4'-hydroxy-substituted flavonoids, quercetin, luteolin, taxifolin and (+)-catechin. The presence of flavonoids decreased binding constants of pantoprazole with BSA from 39.7% to 93.8%, which depended on flavonoid structures. Analysis of(More)
Though taurine has been reported very useful in preventing oxidative stress and various age-related diseases, the detailed biochemical mechanism of its biological functions is not well understood. Direct reaction of malondialdehyde (MDA) with taurine was studied using spectrofluorometry, spectrophotometry and liquid chromatography online with mass(More)
Traditional activity-guided fractionation of natural products is a time-consuming, labor intensive, and expensive strategy, which cannot compete with high-throughput and rapid screening of natural products. Therefore, more efficient approaches are necessary for searching active compounds from natural products. Three main methods based on high-performance(More)