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This paper presents my personal account of research on CO 2 fixation from when I began these studies as a postdoctoral student in the early 1970s. It traces interests in microbial ribulose bisphosphate carboxylase/oxygenase (Rubisco) and considers early breakthroughs on the isolation, characterization, and significance of this enzyme from nonsulfur purple(More)
Syn-and anti-benzo[a]pyrene diol epoxides elicit a concentration-dependent nicking of superhelical Col E1 DNA in an in vitro reaction monitored by agarose gel electrophoresis and electron microscopy. This strand scission represents less than 1 percent of the DNA modification by diol epoxide. Kinetic analysis implicates the formation of unstable(More)
Introduction: Over the last two Earth years the Mars Exploration Rover Opportunity has investigated, via remote sensing and in situ observations, an extensive occurrence of hematite and sulfate-salt rich sedimentary bedrock exposures in crater walls and plains surfaces [1]. Farrand et al. [2] have described the major spectral properties of bedrock in the(More)
A simple method is described for the freeze-fracture in situ of monolayer cultures grown on gold carriers coated with a thin layer of silicon monoxide. Preliminary observations on 3T3 mouse embryo fibroblasts indicate that this technique exposes large areas of cell membrane, making it possible to determine how areas of membrane specialization are related to(More)