Melinda Marshall

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Autoantibodies to glutamic acid decarboxylase (GAD) are frequent at or before the onset of insulin-dependent diabetes mellitus (IDDM). We have developed a simple, reproducible, and quantitative immunoprecipitation radioligand assay using as antigen in vitro transcribed and translated [35S]methionine-labeled human islet GAD65. By using this assay, 77% (77 of(More)
Family history, as well as genetic and immunological markers of diabetes mellitus, were studied in cystic fibrosis (CF) patients with and without diabetes mellitus. Positive family history of diabetes mellitus in first-degree relatives was found in only 6 of 210 (3%) CF patients, with no difference between non-diabetic and diabetic patients. The frequency(More)
The GABA synthesizing enzyme GAD is a prominent islet cell autoantigen in type I diabetes. The two forms of GAD (GAD64 and GAD67) are encoded by different genes in both rats and humans. By in situ hybridization analysis of rat and human pancreases, expression of both genes was detected in rat islets, whereas only GAD64 mRNA was detected in human islets.(More)
The 1-acylglycerolphosphate actyltransferase from a microsomal fraction of lactating cow mammary gland was active towards acyl-CoAs of chain length C8-C18, but not towards butyryl-CoA or hexanoyl-CoA. The lack of activity towards butyryl-CoA and hexanoyl-CoA explains why butyric and hexanoic acid are largely excluded from the sn-2 position of(More)
Mumps epidemics are followed by sporadic cases of insulin dependent diabetes mellitus (IDDM). We have studied beta-cell function in 11 subjects who had had a mumps infection. They had no clinical pancreatitis but were selected as they had abnormal pancreas iso-amylase values and/or glucosuria during the mumps virus infection. At the follow-up some years(More)
We present ERM-Tutor, a constraint-based tutor that teaches logical database design (i.e. mapping conceptual to logical database schemas). Students practice this procedural task in ERM-Tutor by solving each step and receiving feedback on their solutions. We also present a new feature added to the system, which enables students to ask free-form questions. A(More)
1. The effects of medium glucose concentration (0-20 mmol/l) and flow (100-33% of normal) on lactate uptake at low lactate concentration were studied in perfused livers from 48-h-starved rats with perfusate pH values of 7.4 and 6.8. 2. Lactate uptake was independent of glucose concentration in the range 5-10 mmol/l, but was slightly inhibited with time at(More)
The effect of pH on lactate uptake was studied in perfused liver of rats starved for 48 h. At both low pH (6.8) and normal pH (7.4) lactate uptake was a linear function of lactate concentration in input medium in the range 0.4-1.5 mmol/l. In the lower concentration range (0.4-0.8 mmol/l) the rate of lactate uptake was 30% higher at pH 6.8 than at pH 7.4. At(More)