Melanie E Jones

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Aspartate transcarbamylase from a Pseudomonas fluerescens has been purified to homogeneity. The enzyme has a molecular weight of 360,000. It is composed of 2 apparently identical subunits with molecular weights of 180,000. Activity can be recovered from the enzyme denatured with mercaptoethanol and sodium dodecyl sulfate when the dodecyl sulfate is dialyzed(More)
The oxidation of proline to glutamate in mitochondria requires two enzymes, proline oxidase and pyrroline 5-carboxylate (P5C) dehydrogenase. In this paper we report an 800-fold purification P5C dehydrogenase from rat liver mitochondria to yield an essentially homogenous protein. The protein, whose Mr is 59,000, is an alpha 2 dimer (Mr = 115,000) in solution(More)
In vitro incubations (6 hr at 37 degrees C) of human low density lipoproteins (LDL), high density lipoproteins (HDL), and lipoprotein-free plasma revealed no significant net mass transfers of esterified cholesterol from either lipoprotein fraction to the other. Transfers of esterified [3H]cholesterol from LDL to HDL must therefore have represented a process(More)
Exchanges of esterified cholesterol between human high density lipoproteins (HDL) and very low density lipoproteins (VLDL) and between VLDL and low density lipoproteins (LDL) have been fitted to a mathematical model previously developed to describe exchanges between human HDL and LDL. In all cases the fit of the model predicted exchanges to those measured(More)