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The oxidation of proline to glutamate in mitochondria requires two enzymes, proline oxidase and pyrroline 5-carboxylate (P5C) dehydrogenase. In this paper we report an 800-fold purification P5C dehydrogenase from rat liver mitochondria to yield an essentially homogenous protein. The protein, whose Mr is 59,000, is an alpha 2 dimer (Mr = 115,000) in solution(More)
Human uridine monophosphate (UMP) synthase, a bifunctional protein containing orotate phsophoribosyltransferase (OPRTase, EC 2.4.2.10) and orotidine 5'-monophosphate decarboxylase (ODCase, EC 4.1.1.23) activities, has been overproduced by construction and use of a recombinant baculovirus containing the cDNA for this protein. Expression of the virus in(More)
We have studied factors affecting the catalytic activity of dihydroorotase (EC 3.5.2.3), purified as part of a multienzymatic protein which contains carbamyl phosphate synthetase, aspartate transcarbamylase, and dihydroorotase (ME pyr1-3) and which initiates de novo pyrimidine biosynthesis in mouse Ehrlich ascites carcinoma. The apparent Km value for(More)