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In recent years, the potential value of the non-structural proteins (NSP) 2C and 3ABC has been well documented for differentiation of animals infected with foot-and-mouth disease virus (FMDV) from vaccinated animals (DIVA). In order to develop a more sensitive approach to detect animals infected naturally in herds of FMDV-vaccinated animals, a 47.6kD fusion(More)
A monoclonal antibody (McAb) against non-structural protein (NSP) 3B of foot-mouth-disease virus (FMDV) (3B4B1) was generated and shown to recognize a conserved epitope spanning amino acids 24-32 of 3B (GPYAGPMER) by peptide screening ELISA. This epitope was further shown to be a unique and predominant B cell epitope in 3B2, as sera from animals infected(More)
In recent years, the potential value of nonstructural protein (NSP) 2C was well documented for distinguishing foot-and-mouth disease virus (FMDV) in infected animals and vaccinated animals. In order to develop a more sensitive approach to detect natural infected FMDV while there is no interact with vaccinated FMDV, we incorporated a major epitope region of(More)
To explore aminoguanidine (AG) effect on neutrophil functions and associated signal transduction way in diabetic rats wound healing. Sprague-Dawley (SD) rats were divided into 3 groups, Group A (control+burns), Group B (diabetes+burns), Group C (diabetes+burns+AG). Wound skin tissue was harvested at 6h, 24h and 36h after trauma, and then(More)
A novel nanocomposite of CdTe-PAMAM-MWNT was synthesized by covalently linking CdTe quantum dots (QDs) onto highly water-soluble multi-wall carbon nanotubes (MWNTs) functionalized with dendritic poly(amidoamine) (PAMAM). The IR, UV-vis and TEM methods has been used for the characterization of the composites. A facile method for controlling the density of(More)
OBJECTIVE To purify and to detect reactivity of non-structural proteins 3A, 3B and 2C expressed in the Escherichia coli. METHODS FMDV NSP 3A, 3B and 2C containing the major B-cell antigenic sites were expressed in E. coli. We got renatured 2C protein by lysing of isolated inclusion body using high concentration of urea, and then diluted in a buffer system(More)
This paper reports the result of experiment of carbon agglutination test used for the diagnosis of Rotavirus. The results of our experiments demonstrate that this method has many advantages, such as simplicity, rapidity and specificity. One hundred and five stool samples were detected by carbon agglutination test and polyacrylamidegel electrophoresis. The(More)
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